Publication Details |
| Category | Software Publication |
| DOI | 10.5281/zenodo.11396730 |
| Licence |
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| Title (Primary) | Analysis of Zebrafish Swimming Behavior Data using Mixed Effects Modelling |
| Version | v0.1 |
| Author | Gutsfeld, S. |
| Source Titel | Zenodo |
| Year | 2024 |
| Department | ETOX |
| Topic | T9 Healthy Planet |
| Abstract | We hypothesized that host-associated microorganisms can alter host behavior by modifying perfluorooctane sulfonamides to produce perfluorooctanesulfonic acid (PFOS) or other potentially neuroactive metabolites. Zebrafish larvae (Danio rerio) were exposed to PFOS (0.28–5 μM), N-ethyl perfluorooctane sulfonamide (N-EtFOSA, 0.07–1.25 μM), or perfluorooctane sulfonamido ammonium iodide (PFOSAmS, 0.83–15 μM) from 5 to 6 days post fertilization (dpf). This resulted in altered dark-phase swimming behavior at 8 dpf. Exposure to PFOS, or N-EtFOSA, but not PFOSAmS caused a similar shift in community structure. We exposed microbiome-depleted (axenic, AX), conventionally colonized (CC), or axenic conventionalized on day 1 (AC1) zebrafish larvae to N-EtFOSA (0.22–0.7 μM) from 5 to 6 dpf. In comparison to CC and AC1 larvae, AX-larvae did not exhibit concentration-dependent hypoactivity at 8 dpf. Chemical analysis at 7 dpf revealed a significant increase in N-EtFOSA levels in AX-larvae and AX-water samples, relative to colonized cohorts. The main metabolite detected was perfluorooctane sulfonamide (FOSA), which was not microbiome-dependent. Perfluorooctane sulfonamidoacetic acid (FOSAA) was detected at lower levels in AX-larvae, relative to colonized groups. This study revealed that the presence of the microbiome enhanced N-EtFOSA-dependent behavior effects at the level of the host. This supports the concept that chemical–microbiome interactions can influence host phenotypic outcomes. |
| linked UFZ text publications | |
| Gutsfeld, S. (2024): Analysis of Zebrafish Swimming Behavior Data using Mixed Effects Modelling Version: v0.1 Zenodo 10.5281/zenodo.11396730 |
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