Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1021/acs.est.5c16330 |
Licence  |
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| Title (Primary) | N-Ethyl perfluorooctane sulfonamide (N-EtFOSA) exposure alters microbiome composition and causes microbiome-dependent behavior effects in larval zebrafish |
| Author | Gutsfeld, S.; Wray, C.; Schweiger, N.; Röhrig, A.; Paschke, H.; Fu, Q.; Kasmanas, J.C.; Abdulkadir, N.; Kader, S.; Rocha, U.; Ebert, A.
; Tal, T.
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| Source Titel | Environmental Science & Technology |
| Year | 2026 |
| Department | ETOX; COMPBC; EAC |
| Volume | 60 |
| Issue | 20 |
| Page From | 14276 |
| Page To | 14291 |
| Language | englisch |
| Topic | T9 Healthy Planet T7 Bioeconomy |
| Data and Software links | https://doi.org/10.5281/zenodo.11396730 |
| Supplements | Supplement 1 |
| Keywords | biotransformation; chemical–microbiome interactions; behavior; gut-brain-axis; PFAS; bacteria; toxicokinetics; toxicodynamics |
| Abstract | We hypothesized that host-associated microorganisms can alter host behavior by modifying perfluorooctane sulfonamides to produce perfluorooctanesulfonic acid (PFOS) or other potentially neuroactive metabolites. Zebrafish larvae (Danio rerio) were exposed to PFOS (0.28–5 μM), N-ethyl perfluorooctane sulfonamide (N-EtFOSA, 0.07–1.25 μM), or perfluorooctane sulfonamido ammonium iodide (PFOSAmS, 0.83–15 μM) from 5 to 6 days post fertilization (dpf). This resulted in altered dark-phase swimming behavior at 8 dpf. Exposure to PFOS, or N-EtFOSA, but not PFOSAmS caused a similar shift in community structure. We exposed microbiome-depleted (axenic, AX), conventionally colonized (CC), or axenic conventionalized on day 1 (AC1) zebrafish larvae to N-EtFOSA (0.22–0.7 μM) from 5 to 6 dpf. In comparison to CC and AC1 larvae, AX-larvae did not exhibit concentration-dependent hypoactivity at 8 dpf. Chemical analysis at 7 dpf revealed a significant increase in N-EtFOSA levels in AX-larvae and AX-water samples, relative to colonized cohorts. The main metabolite detected was perfluorooctane sulfonamide (FOSA), which was not microbiome-dependent. Perfluorooctane sulfonamidoacetic acid (FOSAA) was detected at lower levels in AX-larvae, relative to colonized groups. This study revealed that the presence of the microbiome enhanced N-EtFOSA-dependent behavior effects at the level of the host. This supports the concept that chemical–microbiome interactions can influence host phenotypic outcomes. |
| Gutsfeld, S., Wray, C., Schweiger, N., Röhrig, A., Paschke, H., Fu, Q., Kasmanas, J.C., Abdulkadir, N., Kader, S., Rocha, U., Ebert, A., Tal, T. (2026): N-Ethyl perfluorooctane sulfonamide (N-EtFOSA) exposure alters microbiome composition and causes microbiome-dependent behavior effects in larval zebrafish Environ. Sci. Technol. 60 (20), 14276 - 14291 10.1021/acs.est.5c16330 |
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