Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.electacta.2020.137326
Document author version
Title (Primary) Electroactive microorganisms in mouse feces
Author Rago, L.; Popp, D.; Heiker, J.T.; Harnisch, F. ORCID logo
Source Titel Electrochimica Acta
Year 2021
Department UMB
Volume 365
Page From art. 137326
Language englisch
Topic T7 Bioeconomy
Keywords Extracellular Electron Transfer (EET); Gut microbiome; Electroactive microorganisms; Next generation sequencing (NGS); Whole genome sequencing (WGS)
Abstract The gut microbiome is not only an indicator of different pathologies, but it also influences metabolism and overall health of the host. Recently, microorganisms inherent to the gut microbiome, such as Listeria monocytogenes, Enterococcus faecalis and Clostridium cochlearium, were demonstrated to be electroactive, i.e. to perform extracellular electron transfer (EET). To further explore the presence of electroactive microorganisms in the gut microbiome electrochemical enrichment starting from mouse feces was performed. Open circuit, abiotic and autoclaved inoculum controls were run in parallel. A maximum current density of 122±23 µA cm−2 at low coulombic efficiency ( ̴1%) was achieved. The presence of biofilms at the anode and microbial electrochemical activity with a formal potential of EET of 0.23±0.01 V vs. Ag/AgCl sat. KCl was demonstrated using fluorescence microscopy and cyclic voltammetry. The 16S rRNA gene sequencing and PCR-free Nanopore sequencing showed the enrichment and dominance of Shigella flexneri.
Persistent UFZ Identifier
Rago, L., Popp, D., Heiker, J.T., Harnisch, F. (2021):
Electroactive microorganisms in mouse feces
Electrochim. Acta 365 , art. 137326 10.1016/j.electacta.2020.137326