Publication Details

Reference Category Journals
DOI / URL link
Document accepted manuscript
Title (Primary) Tracking microbial interactions with NanoSIMS
Author Musat, N.; Musat, F.; Weber, P.K.; Pett-Ridge, J.;
Journal Current Opinion in Biotechnology
Year 2016
Department ISOBIO;
Volume 41
Language englisch;
POF III (all) T15; T41;
UFZ wide themes RU4;
Abstract The combination of stable isotope probing (SIP), NanoSIMS imaging and microbe identification via fluorescence in situ hybridization (FISH) is often used to link identity to function at the cellular level in microbial communities. Many opportunities remain for nanoSIP to identify metabolic interactions and nutrient fluxes within syntrophic associations and obligate symbioses where exchanges can be extremely rapid. However, additional data, such as genomic potential, gene expression or other imaging modalities are often critical to deciphering the mechanisms underlying specific interactions, and researchers must keep sample preparation artefacts in mind. Here we focus on recent applications of nanoSIP, particularly where used to track exchanges of isotopically labelled molecules between organisms. We highlight metabolic interactions within syntrophic consortia, carbon/nitrogen fluxes between phototrophs and their heterotrophic partners, and symbiont–host nutrient sharing.
ID 17713
Persistent UFZ Identifier
Musat, N., Musat, F., Weber, P.K., Pett-Ridge, J. (2016):
Tracking microbial interactions with NanoSIMS
Curr. Opin. Biotechnol. 41 , 114 - 121