Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.copbio.2016.06.007
Volltext Akzeptiertes Manuskript
Titel (primär) Tracking microbial interactions with NanoSIMS
Autor Musat, N.; Musat, F.; Weber, P.K.; Pett-Ridge, J.
Quelle Current Opinion in Biotechnology
Erscheinungsjahr 2016
Department ISOBIO
Band/Volume 41
Seite von 114
Seite bis 121
Sprache englisch
UFZ Querschnittsthemen RU4;
Abstract The combination of stable isotope probing (SIP), NanoSIMS imaging and microbe identification via fluorescence in situ hybridization (FISH) is often used to link identity to function at the cellular level in microbial communities. Many opportunities remain for nanoSIP to identify metabolic interactions and nutrient fluxes within syntrophic associations and obligate symbioses where exchanges can be extremely rapid. However, additional data, such as genomic potential, gene expression or other imaging modalities are often critical to deciphering the mechanisms underlying specific interactions, and researchers must keep sample preparation artefacts in mind. Here we focus on recent applications of nanoSIP, particularly where used to track exchanges of isotopically labelled molecules between organisms. We highlight metabolic interactions within syntrophic consortia, carbon/nitrogen fluxes between phototrophs and their heterotrophic partners, and symbiont–host nutrient sharing.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17713
Musat, N., Musat, F., Weber, P.K., Pett-Ridge, J. (2016):
Tracking microbial interactions with NanoSIMS
Curr. Opin. Biotechnol. 41 , 114 - 121 10.1016/j.copbio.2016.06.007