Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Volltext Akzeptiertes Manuskript
Titel (primär) Tracking microbial interactions with NanoSIMS
Autor Musat, N.; Musat, F.; Weber, P.K.; Pett-Ridge, J.;
Journal / Serie Current Opinion in Biotechnology
Erscheinungsjahr 2016
Department ISOBIO;
Band/Volume 41
Sprache englisch;
POF III (gesamt) T15; T41;
UFZ Querschnittsthemen RU4;
Abstract The combination of stable isotope probing (SIP), NanoSIMS imaging and microbe identification via fluorescence in situ hybridization (FISH) is often used to link identity to function at the cellular level in microbial communities. Many opportunities remain for nanoSIP to identify metabolic interactions and nutrient fluxes within syntrophic associations and obligate symbioses where exchanges can be extremely rapid. However, additional data, such as genomic potential, gene expression or other imaging modalities are often critical to deciphering the mechanisms underlying specific interactions, and researchers must keep sample preparation artefacts in mind. Here we focus on recent applications of nanoSIP, particularly where used to track exchanges of isotopically labelled molecules between organisms. We highlight metabolic interactions within syntrophic consortia, carbon/nitrogen fluxes between phototrophs and their heterotrophic partners, and symbiont–host nutrient sharing.
ID 17713
dauerhafte UFZ-Verlinkung
Musat, N., Musat, F., Weber, P.K., Pett-Ridge, J. (2016):
Tracking microbial interactions with NanoSIMS
Curr. Opin. Biotechnol. 41 , 114 - 121