ProMetheus- Metabolomics & Proteomics

ProMetheus- Platform for metabolomics analysis

Prometheus is a platform that summarises the application of techniques and methods used to analyse functional relationships at the molecular level using mass spectrometry. Our mission is to adapt methods to new research questions and to apply them within the framework of scientific collaborations. We analyse the effects of chemicals on cells (eukaryotic and prokaryotic), model systems (mouse, rat, bee, in vitro bioreactors) and humans. In particular, the focus is on the elucidation of microbiome-host interactions, which can play a decisive role in the development of functional disorders as the basis for the development of diseases.

Prometheus platform for analysing the metabolome
Both targeted (quantification of known analytes) and non-targeted metabolomics (metabolic profiling, fingerprinting) are used to analyse phenotypic differences at the metabolomic level. A broad portfolio of mass spectrometers is available for this purpose. These include 3 LC-MS-MS systems (Q-Traps, SCIEX), 2 GC-MS systems and a QToF system (Agilent) as well as an Orbitrap IDX mass spectrometer (Thermo Fisher).

Prometheus platform for analysing the proteome
Global proteomics using label-free and label-based quantification is used to investigate phenotypic differences at the proteome level. Two QExactives and an Exploris240 (Thermo Fisher) are available for this purpose.