Spatial distribution of chemicals in aquatic organisms

Key parameters for adverse effects of chemicals to organisms are uptake by and distribution within the organism leading to the “internal exposure” as pivotal factor for biological effects of chemicals and therefore crucial component of the exposome. In order to understand and predict effects of chemicals on organisms, toxicokinetic/toxicodynamic (TKTD) models are required. However, development of these models relies on internal concentration data. A major problem for these internal concentration data is the lack of spatial information. With a focus on the zebrafish embryo (ZFE) – the project aims at improving the mechanistic understanding on uptake of chemicals, resolve toxicokinetics on a spatio-temporal level and establish a link to biological effects. The objectives of this project are:

  1. Development and application of analytical tools for determination of spatial distribution of concentration in the ZFE model.
  2. Improvement and validation of toxicokinetic models for prediction of compound uptake and distribution based on spatial concentration data.
  3. Link of biodistribution to biologic effects.

Project team at the Department Analytics
Katharina Halbach (PhD student)
Stephan Wagner
Thorsten Reemtsma

In collaboration with
Kai-Uwe Goss (Department Analytische Umweltchemie)
Stefan Scholz (Department Bioanalytische Ökotoxikologie)
Till Luckenbach (Department Bioanalytische Ökotoxikologie)