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Title (Primary) Isotopic fingerprinting of methane and CO2 formation from aliphatic and aromatic hydrocarbons
Author Feisthauer, S.; Siegert, M.; Seidel, M.; Richnow, H.H.; Zengler, K.; Gründger, F.; Krüger, M.;
Journal Organic Geochemistry
Year 2010
Department ISOBIO;
Volume 41
Issue 5
Language englisch;
Abstract We investigated the stable carbon and hydrogen isotopic signatures of methane, CO2 and water during microbial formation of methane from mineral oil-related compounds in order to determine the variability in the methane signatures. The isotopic discrimination for carbon and hydrogen between substrate and methane was calculated and resulted in 26-60?, 16-33?, 257-336? and 174-318?, respectively. These carbon and hydrogen isotope signatures fell in to a relatively narrow range, suggesting a coupling of fermentation with acetoclastic and CO2 reducing methanogenesis. In order to characterize the microbial consortia involved in the methanogenic degradation of hexadecane, a methanogenic enrichment culture was incubated with 1-13C-hexadecane and its biomass was analyzed for the pattern and isotopic signature of carboxylic acids. The highest extent of labelling was detected in the n-C17 fatty acid with methyl groups at C-4, presumably indicative of Syntrophus sp. To determine if the isotope composition of methane can be used as an indicator for methanogenesis during growth with oil-related compounds in field studies, we analyzed the isotope composition of methane in a confined mineral oil contaminated aquifer. The variability in carbon and hydrogen isotope composition was almost identical to the values obtained from enrichment cultures, thereby providing a tool for screening for microbial methane formation during hydrocarbon exploration.
ID 9932
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Feisthauer, S., Siegert, M., Seidel, M., Richnow, H.H., Zengler, K., Gründger, F., Krüger, M. (2010):
Isotopic fingerprinting of methane and CO2 formation from aliphatic and aromatic hydrocarbons
Org. Geochem. 41 (5), 482 - 490