Publication Details |
Category | Text Publication |
Reference Category | Journals |
DOI | 10.1002/(SICI)1521-4028(199809)38:4<269::AID-JOBM269>3.0.CO;2-P |
Title (Primary) | Degradation of various chlorophenols under alkaline conditions by Gram-negative bacteria closely related to Ochrobactrum anthropi |
Author | Müller, R.H.; Jorks, S.; Kleinsteuber, S.
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Source Titel | Journal of Basic Microbiology |
Year | 1998 |
Department | UMB |
Volume | 38 |
Issue | 4 |
Page From | 269 |
Page To | 281 |
Language | englisch |
Abstract | From concrete debris of a demolished herbicide production plant several Gram-negative bacterial strains were isolated, which exhibit metabolic capabilities for the degradation of 2,4-dichlorophenol (DCP), 4-chloro-2-methylphenol (MCP) and 4-chlorophenol (4-CP), while 2-chlorophenol (2-CP) was degraded at a slower rate. Degradative activity was inducible and was impeded by adding of 100 mg/l of chloramphenicol to growing cultures. The strains displayed alkaliphilic properties with optimum DCP/MCP degradation at pH values around 8.5—9.5; activity was observed up to pH values of 11. Degradation was most likely complete according to chlorine balances; formation of intermediary products was observed with MCP some time. Specific activity of up to 380 μmol/h · g dry mass was found within the concentration range of 10—20 mg/l DCP; higher concentrations retarded the activity with complete inhibition at 200—400 mg/l. Some of the strains carry plasmids whose presence was not unambiguously correlated to the degradative properties. Ribotyping revealed a high degree of relationship between the strains. Preliminary taxonomic investigations showed close rela-tionship to Ochrobactrum anthropi. |
Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=8869 |
Müller, R.H., Jorks, S., Kleinsteuber, S., Babel, W. (1998): Degradation of various chlorophenols under alkaline conditions by Gram-negative bacteria closely related to Ochrobactrum anthropi J. Basic Microbiol. 38 (4), 269 - 281 10.1002/(SICI)1521-4028(199809)38:4<269::AID-JOBM269>3.0.CO;2-P |