Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/S0021-9673(98)00702-X
Title (Primary) Stability and determination of aflatoxins by high-performance liquid chromatography with amperometric detection
Author Elizalde-González, M.P.; Mattusch, J.; Wennrich, R.
Source Titel Journal of Chromatography A
Year 1998
Department ANA
Volume 828
Issue 1-2
Page From 439
Page To 444
Language englisch
Keywords aflatoxins; electrochemical detection
Abstract

A method based on reversed-phase high-performance liquid chromatography (RP-HPLC) with amperometric detection with a glassy carbon electrode at a constant potential of 1.4 V is reported for the separation and identification of aflatoxins B1, B2, G1 and G2 in a model mixture. The chromatography was performed on a PAH-Baker column with a ternary mobile phase containing methanol, acetonitrile and aqueous LiClO4 electrolyte. Aflatoxin G1 showed the highest electroactivity in the compound series studied. Calibration curves of aflatoxins G1 and B2 were linear up to 0.2 and 0.3 mmol/l, respectively. Sensitivity varied between 7 and 10 ng for the different aflatoxins. The combination of different HPLC detectors in the analysis of these compounds was applied to investigate the stability of aflatoxins G1 and B2.

Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=8610
Elizalde-González, M.P., Mattusch, J., Wennrich, R. (1998):
Stability and determination of aflatoxins by high-performance liquid chromatography with amperometric detection
J. Chromatogr. A 828 (1-2), 439 - 444 10.1016/S0021-9673(98)00702-X