Publication Details

Category Text Publication
Reference Category Book chapters
DOI 10.1016/S0076-6879(99)10012-0
Title (Primary) Lectin-binding analysis in biofilm systems
Title (Secondary) Biofilms
Author Neu, T.R.; Lawrence, J.R.
Publisher Doyle, R.J.
Journal Methods in Enzymology
Year 1999
Department FLOEK
Volume 310
Page From 145
Page To 152
Language englisch
Abstract This chapter discusses the lectin-binding analysis in biofilm systems. The difficulty of isolating a single polymer type from a complex biofilm matrix may be comparable to the situation at the cellular level. As a consequence, there is a need to establish an in situ technique for the assessment of glycoconjugate distribution in biofilm systems. At present, the most promising approach to achieve this is the application of lectin-binding analyses in combination with confocal laser scanning microscopy (CLSM). The applicability of lectins to probe living and fully hydrated biofilm systems is based on the definition of biofilms. A general definition for biofilms is the collection of microorganisms and their extracellular polymeric substances (EPS) associated with an interface. Extracellular polymeric substances are defined as organic polymers of biological origin, which in biofilm systems are responsible for the interaction with interfaces. The EPS may include polysaccharides, proteins, nucleic acids, and amphiphilic polymeric compounds. Thus, in biofilm systems, one can expect two types of polymeric carbohydrate structures: (1) those located on cell surfaces and (2) those located extracellularly throughout the biofilm matrix.
Persistent UFZ Identifier
Neu, T.R., Lawrence, J.R. (1999):
Lectin-binding analysis in biofilm systems
In: Doyle, R.J. (ed.)
Methods Enzymol. 310
Elsevier, p. 145 - 152