Publication Details

Category Text Publication
Reference Category Book chapters
DOI 10.1016/S0076-6879(99)10011-9
Title (Primary) Confocal laser scanning microscopy for analysis of microbial biofilms
Title (Secondary) Biofilms
Author Lawrence, J.R.; Neu, T.R.
Publisher Doyle, R.J.
Source Titel Methods in Enzymology
Year 1999
Department FLOEK
Volume 310
Page From 131
Page To 144
Language englisch
Abstract Among the most versatile and effective of the nondestructive approaches for studying biofilms is confocal laser scanning microscopy (CLSM). CLSM reduces greatly the need for pretreatments such as disruption and fixation that reduce or eliminate the evidence for microbial relationships, complex structure, and organization in biofilms. CLSM has also been used extensively in combination with fluorescent in situ hybridization techniques. CLSM provides a digital database that is amenable to image processing and analysis. Thus the user may obtain quantitative information on a wide variety of parameters, including cell numbers, cell area, object parameters such as minimum/maximum dimensions, orientation, and average gray value. This chapter aims to provide a primer for CLSM observation of biofilm materials. CLSM offers the only means for real-time, in-depth analysis of undisturbed biofilms.
Persistent UFZ Identifier
Lawrence, J.R., Neu, T.R. (1999):
Confocal laser scanning microscopy for analysis of microbial biofilms
In: Doyle, R.J. (ed.)
Methods Enzymol. 310
Elsevier, p. 131 - 144 10.1016/S0076-6879(99)10011-9