Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/S0269-7491(99)00205-5
Title (Primary) Tracing the transformation of labelled [1-13C]phenanthrene in a soil bioreactor
Author Richnow, H.H.; Annweiler, E.; Koning, M.; Lüth, J.C.; Stegmann, R.; Garms, C.; Franke, W.; Michaelis, W.
Source Titel Environmental Pollution
Year 2000
Department ISOBIO
Volume 108
Issue 1
Page From 91
Page To 101
Language englisch
Keywords transformation; 13C-label; phenanthrene; soil bioreactor

[1-13C]-labelled phenanthrene was incubated in a closed bioreactor to study the flux and biotransformation of polycyclic aromatic hydrocarbon (PAH) in contaminated soils on a bulk and molecular level. The degradation of extractable phenanthrene was observed by GC–MS measurements and the mineralisation was monitored by 13CO2 production. The transformation of the 13C-label into non-extractable soil-bound residues was determined by carbon isotopic measurements. With these data we were able to calculate a carbon budget of the 13C-label. Moreover, the chemical structure of non-extractable bound residues was characterised by applying selective chemical degradation reactions to cleave xenobiotic subunits from the macromolecular organic soil matrix. The obtained low molecular weight products yielded 13C-labelled compounds which were identified using IRM (isotope ratio monitoring)–GC–MS and structurally characterised with GC–MS. Most of the 13C-labelled products obtained by chemical degradation of non-extractable bound residues are well-known metabolites of phenanthrene. Thus, metabolites of [1-13C]phenanthrene formed during biodegradation appear to be reactive components which are subsequently involved in the bound residue formation. Hydrolysable amino acids of the soil residues were significantly labelled with 13C as confirmed by IRM–GC–MS measurements. Therefore, phenanthrene-derived carbon was transformed by anabolic microbial processes into typical biologically derived compounds. These substances are likely to be incorporated into humic-like material after cell death.

Persistent UFZ Identifier
Richnow, H.H., Annweiler, E., Koning, M., Lüth, J.C., Stegmann, R., Garms, C., Franke, W., Michaelis, W. (2000):
Tracing the transformation of labelled [1-13C]phenanthrene in a soil bioreactor
Environ. Pollut. 108 (1), 91 - 101 10.1016/S0269-7491(99)00205-5