Publication Details |
Reference Category | Journals |
DOI / URL | link |
Title (Primary) | Anaerobic naphthalene degradation by a sulfate-reducing enrichment culture |
Author | Meckenstock, R.U.; Annweiler, E.; Michaelis, W.; Richnow, H.H. |
Journal | Applied and Environmental Microbiology |
Year | 2000 |
Department | ISOBIO |
Volume | 66 |
Issue | 7 |
Page From | 2743 |
Page To | 2747 |
Language | englisch |
Keywords | polycyclic aromatic-hydrocarbons; denitrifying bacterium; initial reactions; o-xylene; toluene; oxidation; metabolism; benzylsuccinate; activation; reduction |
Abstract |
Anaerobic naphthalene degradation by a sulfate-reducing enrichment culture was studied by substrate utilization tests and identification of metabolites by gas chromatography-mass spectrometry. In substrate utilization tests, the culture was able to oxidize naphthalene, 2-methylnaphthalene, 1- and 2-naphthoic acids, phenylacetic acid, benzoic acid, cyclohexanecarboxylic acid, and cyclohex-1-ene-carboxylic acid with sulfate as the electron acceptor. Neither hydroxylated 1- or 2-naphthoic acid derivatives and 1- or 2-naphthol nor the monoaromatic compounds ortho-phthalic acid, 2-carboxy-1-phenylacetic acid, and salicylic acid were utilized by the culture within 100 days. 2-Naphthoic acid accumulated in all naphthalene-grown cultures. Reduced 2-naphthoic acid derivatives could be identified by comparison of mass spectra and coelution with commercial reference compounds such as 1,2,3,4-tetrahydro-2-naphthoic acid and chemically synthesized decahydro-2-naphthoic acid. 5,6,7,8-Tetrahydro-2-naphthoic acid and octahydro-2-naphthoic acid were tentatively identified by their mass spectra. The metabolites identified suggest a stepwise reduction of the aromatic ring system before ring cleavage. In degradation experiments with [1-13C]naphthalene or deuterated D8-naphthalene, all metabolites mentioned derived from the introduced labeled naphthalene. When a [13C]bicarbonate-buffered growth medium was used in conjunction with unlabeled naphthalene, 13C incorporation into the carboxylic group of 2-naphthoic acid was shown, indicating that activation of naphthalene by carboxylation was the initial degradation step. No ring fission products were identified. |
Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=7420 |
Meckenstock, R.U., Annweiler, E., Michaelis, W., Richnow, H.H. (2000): Anaerobic naphthalene degradation by a sulfate-reducing enrichment culture Appl. Environ. Microb. 66 (7), 2743 - 2747 |