Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/S0043-1354(99)00123-2
Title (Primary) Performance and microbial structure of a nitrifying fluidized-bed reactor
Author Kloep, F.; Röske, I.; Neu, T.R.
Journal Water Research
Year 2000
Department FLOEK; GM
Volume 34
Issue 1
Page From 311
Page To 319
Language englisch
Keywords biofilm; confocal laser scanning microscopy; fluidized bed reactor; fluorescent in situ hybridization; nitrifying bacteria; wastewater
Abstract The performance and composition of the microbial community have been examined in a nitrifying fluidized-bed biofilm reactor for wastewater treatment arranged as the ultimate treatment step subsequent to BOD- and biological P-elimination. The microbial composition was analyzed by means of rRNA-targeted oligonucleotide probes. An NH4+-N elimination of more than 95% at influent concentrations of up to 80 mg NH4+-N l-1 was observed during a period with 75% carrier material (v/v) and a hydraulic retention time of 1.8 h. Using a confocal laser scanning microscope it was found that the biofilm structure on the carrier material (tubes of 5 mm length) was mainly influenced by the loss processes affecting the biofilm. The microbial composition, using fluorescent in situ hybridization, was dominated by the beta-group of the Proteobacteria (up to 55% of the DAPI cell number). The ammonia-oxidizing bacteria, a subgroup of the beta-Proteobacteria, were characterized by their occurrence in clusters. Using different probes for ammonia-oxidizing bacteria, a shift in the population structure within the experimental period was observed. Nitrobacter could not be detected with probes such as NIT1, NIT2 and NIT3.
Persistent UFZ Identifier
Kloep, F., Röske, I., Neu, T.R. (2000):
Performance and microbial structure of a nitrifying fluidized-bed reactor
Water Res. 34 (1), 311 - 319