Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/S0043-1354(99)00123-2
Title (Primary) Performance and microbial structure of a nitrifying fluidized-bed reactor
Author Kloep, F.; Röske, I.; Neu, T.R.
Source Titel Water Research
Year 2000
Department FLOEK; GM
Volume 34
Issue 1
Page From 311
Page To 319
Language englisch
Keywords biofilm; confocal laser scanning microscopy; fluidized bed reactor; fluorescent in situ hybridization; nitrifying bacteria; wastewater
Abstract The performance and composition of the microbial community have been examined in a nitrifying fluidized-bed biofilm reactor for wastewater treatment arranged as the ultimate treatment step subsequent to BOD- and biological P-elimination. The microbial composition was analyzed by means of rRNA-targeted oligonucleotide probes. An NH4+-N elimination of more than 95% at influent concentrations of up to 80 mg NH4+-N l-1 was observed during a period with 75% carrier material (v/v) and a hydraulic retention time of 1.8 h. Using a confocal laser scanning microscope it was found that the biofilm structure on the carrier material (tubes of 5 mm length) was mainly influenced by the loss processes affecting the biofilm. The microbial composition, using fluorescent in situ hybridization, was dominated by the beta-group of the Proteobacteria (up to 55% of the DAPI cell number). The ammonia-oxidizing bacteria, a subgroup of the beta-Proteobacteria, were characterized by their occurrence in clusters. Using different probes for ammonia-oxidizing bacteria, a shift in the population structure within the experimental period was observed. Nitrobacter could not be detected with probes such as NIT1, NIT2 and NIT3.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=7321
Kloep, F., Röske, I., Neu, T.R. (2000):
Performance and microbial structure of a nitrifying fluidized-bed reactor
Water Res. 34 (1), 311 - 319 10.1016/S0043-1354(99)00123-2