Publication Details

Category Text Publication
Reference Category Book chapters
DOI 10.1016/B978-012529650-2/50047-0
Title (Primary) Cell and tissue culture
Title (Secondary) The laboratory fish
Author Ganassin, R.C.; Schirmer, K.; Bols, N.C.
Publisher Ostrander, G.K.
Source Titel Handbook of Experimental Animals
Year 2000
Department OEC; ZELLTOX; COE
Page From 631
Page To 651
Language englisch
Abstract The culturing, development, and monitoring requirements vary with the goals, cell type, and species under investigation, much information can be obtained from the protocols for developing specific types of fish cell cultures and for monitoring general and specific functions in fish cell cultures. This chapter describes methods for developing primary long-term hemopoietic cultures from fish. An active hemopoietic culture continuously produces blood cells for a period of up to six months. The influence of various additions to the basic culture medium (for example, toxicants, growth factors, and cytokines) provides information about the factors that perturb blood cell formation. The methods of measuring general and specific cell parameters useful in both basic research and aquatic toxicology are outlined in the chapter. These are cell viability and the induction of 7-ethoxyresorufin-o-deethylase (EROD) activity in fish cell cultures. The results of cell viability assays can be measured rapidly and inexpensively in microwell cultures with a multiwell fluorometric plate reader, improving the utility of fish cell cultures in many disciplines but especially in toxicology. The species used is rainbow trout (Oncorhynchus mykiss) because it is the most commonly used species in fish research.
Persistent UFZ Identifier
Ganassin, R.C., Schirmer, K., Bols, N.C. (2000):
Cell and tissue culture
In: Ostrander, G.K. (ed.)
The laboratory fish
Handbook of Experimental Animals
Elsevier, p. 631 - 651 10.1016/B978-012529650-2/50047-0