Publication Details |
| Category | Text Publication |
| Reference Category | Book chapters |
| DOI | 10.1016/B978-012529650-2/50047-0 |
| Title (Primary) | Cell and tissue culture |
| Title (Secondary) | The laboratory fish |
| Author | Ganassin, R.C.; Schirmer, K.; Bols, N.C. |
| Publisher | Ostrander, G.K. |
| Source Titel | Handbook of Experimental Animals |
| Year | 2000 |
| Department | OEC; ZELLTOX; COE |
| Page From | 631 |
| Page To | 651 |
| Language | englisch |
| Abstract | The culturing, development, and monitoring requirements vary with the goals, cell type, and species under investigation, much information can be obtained from the protocols for developing specific types of fish cell cultures and for monitoring general and specific functions in fish cell cultures. This chapter describes methods for developing primary long-term hemopoietic cultures from fish. An active hemopoietic culture continuously produces blood cells for a period of up to six months. The influence of various additions to the basic culture medium (for example, toxicants, growth factors, and cytokines) provides information about the factors that perturb blood cell formation. The methods of measuring general and specific cell parameters useful in both basic research and aquatic toxicology are outlined in the chapter. These are cell viability and the induction of 7-ethoxyresorufin-o-deethylase (EROD) activity in fish cell cultures. The results of cell viability assays can be measured rapidly and inexpensively in microwell cultures with a multiwell fluorometric plate reader, improving the utility of fish cell cultures in many disciplines but especially in toxicology. The species used is rainbow trout (Oncorhynchus mykiss) because it is the most commonly used species in fish research. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=7148 |
| Ganassin, R.C., Schirmer, K., Bols, N.C. (2000): Cell and tissue culture In: Ostrander, G.K. (ed.) The laboratory fish Handbook of Experimental Animals Elsevier, p. 631 - 651 10.1016/B978-012529650-2/50047-0 |
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