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Title (Primary) Different immunomodulatory effects associated with sub-micrometer particles in ambient air from rural, urban and industrial areas
Author Wichmann, G.; Franck, U.; Herbarth, O.; Rehwagen, M.; Dietz, A.; Massolo, L.; Ronco, A.; Müller, A.;
Journal Toxicology
Year 2009
Department IMMU; PROTEOM; STUDIEN; EXPOEPID;
Volume 257
Issue 3
Language englisch;
Keywords Ultrafine particles; Hexane extract; Interleukin-4 (IL-4); Interferon gamma (IFN-gamma); Immune suppression; Lymphocytes; In vitro
Abstract Immunomodulatory effects of chemicals adsorbed to particles with aerodynamic diameter below 0.49 µm (PM0.5) collected in winter 2001 at three sampling points (industrial area [LPIn], traffic-influenced urban area [LPCi], and control area [LPCo]) of La Plata, Argentina, were investigated. The sampling of particulate matter was carried out with high-volume collectors using cascade impactors. PM0.5-adsorbed compounds were hexane-extracted by accelerated solvent extraction. For immunological investigations, human peripheral blood lymphocytes were activated by phytohemagglutinin and exposed to dimethyl-sulfoxide dilutions of PM0.5-extracts for 24 h. Vitality/proliferation was quantified using MTT, released interferon-? (IFN-?) and interleukin-4 (IL-4) by ELISA. Cytokine production but not vitality/proliferation was significantly suppressed by all of the highest extract concentrations. Generally, suppression of IFN-? by PM0.5-extracts was stronger than those of IL-4. Based on administered mass of PM0.5, all extracts suppressed IFN-? production nearly uniform. Contrary, LPCi-extracts exerted maximum IFN-? suppression based either on air volume or regarding PM0.5-adsorbed PAH. Also the ranking of PM0.5-associated effects on IL-4 production differs in dependence of the chosen reference points, either mass or [µg/ml] or air volume [m3/ml] related dust quantities in cell culture. Based on the corresponding air volume, LPCi-extracts inhibited IL-4 production to the maximum extend, whereas suppression of IL-4 was comparable based on concentrations. This indicates that not only the disparate PM0.5-masses in air cause varying impacts, but also that disparities in PM0.5-adsorbed chemicals provoke different effects on immune responses and shifts in the regulatory balance that might have implications for allergy and cancer development.
ID 710
Persistent UFZ Identifier http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=710
Wichmann, G., Franck, U., Herbarth, O., Rehwagen, M., Dietz, A., Massolo, L., Ronco, A., Müller, A. (2009):
Different immunomodulatory effects associated with sub-micrometer particles in ambient air from rural, urban and industrial areas
Toxicology 257 (3), 127 - 136