Publication Details

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Title (Primary) A new medium free of organic carbon to cultivate organisms from extremely acidic mining lakes (pH 2.7)
Author Bissinger, V.; Jander, J.; Tittel, J.;
Journal Acta Hydrochimica et Hydrobiologica
Year 2000
Department SEEFO; GM;
Volume 28
Language englisch;
Keywords Acidic Lake;Culture Medium;Mixotrophy;Plankton;Heterotrophic Potential;ChlamydomonasSaurer See;Kulturmedium;Mixotrophie;Plankton;heterotrophes Potential;Chlamydomonas
Abstract

An algal culture medium was developed which reflects the extreme chemical conditions of acidic mining lakes (pH 2.7, high concentrations of iron and sulfate) and remains stable without addition of organic carbon sources. It enables controlled experiments e.g. on the heterotrophic potential of pigmented flagellates in the laboratory. Various plankton organisms isolated from acidic lakes were successfully cultivated in this medium. The growth rates of a Chlamydomonas isolate from acidic mining lakes were assessed by measuring cell densities under pure autotrophic and heterotrophic conditions (with glucose as organic C-source) and showed values of 0.74 and 0.40, respectively.

ID 6998
Persistent UFZ Identifier http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=6998
Bissinger, V., Jander, J., Tittel, J. (2000):
A new medium free of organic carbon to cultivate organisms from extremely acidic mining lakes (pH 2.7)
Acta Hydrochim. Hydrobiol. 28 , 310 - 312