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Title (Primary) Anaerobic degradation of 2-methylnaphthalene by a sulfate-reducing enrichment culture
Author Annweiler, E.; Materna, A.; Safinowski, M.; Kappler, A.; Richnow, H.H.; Michaelis, W.; Meckenstock, R.U.;
Journal Applied and Environmental Microbiology
Year 2000
Department ISOBIO;
Volume 66
Issue 12
Language englisch;
Keywords polycyclic aromatic-hydrocarbons; denitrifying bacterium; naphthalene degradation; enzymatic reduction; Thauera aromatica; anoxic conditions; key reaction; benzoyl-CoA; strain T; toluene
Abstract

Anaerobic degradation of 2-methylnaphthalene was investigated with a sulfate-reducing enrichment culture. Metabolite analyses revealed two groups of degradation products. The first group comprised two succinic acid adducts which were identified as naphthyl-2-methyl-succinic acid and naphthyl-2-methylene-succinic acid by comparison with chemically synthesized reference compounds. Naphthyl-2-methyl-succinic acid accumulated to 0.5 muM in culture supernatants. Production of naphthyl-2-methyl-succinic acid was analyzed in enzyme assays with dense cell suspensions. The conversion of 2-methylnaphthalene to naphthyl-2-methyl-succinic acid was detected at a specific activity of 0.020 +/- 0.003 nmol min(-1) mg of protein(-1) only in the presence of cells and fumarate. We conclude that under anaerobic conditions 2-methylnaphthalene is activated by fumarate addition to the methyl group, as is the case in anaerobic toluene degradation. The second group of metabolites comprised 2-naphthoic acid and reduced 2-naphthoic acid derivatives, including 5,6,7,8-tetrahydro-2-naphthoic acid, octahydro-2-naphthoic acid, and decahydro-2-naphthoic acid. These compounds were also identified in an earlier study as products of anaerobic naphthalene degradation with the same enrichment culture. A pathway for anaerobic degradation of 2-methylnaphthalene analogous to that for anaerobic toluene degradation is proposed.

ID 6968
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=6968
Annweiler, E., Materna, A., Safinowski, M., Kappler, A., Richnow, H.H., Michaelis, W., Meckenstock, R.U. (2000):
Anaerobic degradation of 2-methylnaphthalene by a sulfate-reducing enrichment culture
Appl. Environ. Microb. 66 (12), 5329 - 5333