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Title (Primary) Producing poly-3-hydroxybutyrate with a high molecular mass from methane
Author Wendlandt, K.-D.; Jechorek, M.; Helm, J.; Stottmeister, U.;
Journal Journal of Biotechnology
Year 2001
Department UBT; UBT_alt; SAN;
Volume 86
Issue 2
Language englisch;
Keywords Poly-3-hydroxybutyrate; Methanotrophs; Methane; Cultivation; Biosynthesis; High molecular mass; Methylocystis sp. GB 25 DSMZ 7674
Abstract Poly-3-hydroxybutyrate (PHB) and other polyesters can be produced by various species of bacteria. Of the possible carbon sources, methane could provide a suitable substrate for the production of PHB. Methane is cheap and plentiful – not only as natural gas, but also as biogas. The methanotrophic strain Methylocystis sp. GB 25 DSMZ 7674 is able to accumulate PHB in a brief non-sterile process. The studies were carried out using a 7-l and a 70-l pressure bioreactor. Cultivation was performed in two stages: a continuous growth phase (dilution rate 0.17 h−1) and a PHB accumulation phase under deficiency conditions of an essential nutrient (ammonium, phosphorus or magnesium) in batch culture. The PHB content of biomass was as high as 51%; efficiency was highest during the first 5 h of the product formation process. The maximum PHB yield relative to the methane consumed was estimated to be 0.55 g g−1. The PHB produced is of very high quality, having a high molecular mass of up to 2.5×106 Da.
ID 6927
Persistent UFZ Identifier
Wendlandt, K.-D., Jechorek, M., Helm, J., Stottmeister, U. (2001):
Producing poly-3-hydroxybutyrate with a high molecular mass from methane
J. Biotechnol. 86 (2), 127 - 133