Publication Details

Reference Category Journals
DOI / URL
Title (Primary) Mutations of tau protein in frontotemporal dementia (FTDP-17) promote aggregation of paired helical filaments by enhancing local β-structure
Author von Bergen, M.; Li, L.; Barghorn, S.; Biernat, J.; Mandelkow, E.-M.; Mandelkow, E.;
Journal Molecular Biology of the Cell
Year 2001
Department PROTEOM;
Volume 12
Issue Suppl. S
Language englisch;
Abstract The microtubule-associated protein tau is a natively unfolded protein in solution, yet it is able to polymerize into the ordered paired helical filaments (PHF) of Alzheimer's disease. In the splice isoforms lacking exon 10, this process is facilitated by the formation of β-structure around the hexapeptide motif PHF6 (306VQIVYK311) encoded by exon 11. We have investigated the structural requirements for PHF polymerization in the context of adult tau isoforms containing four repeats (including exon 10). In addition to the PHF6 motif there exists a related PHF6* motif (275VQIINK280) in the repeat encoded by the alternatively spliced exon 10. We show that this PHF6* motif also promotes aggregation by the formation of β-structure and that there is a cross-talk between the two hexapeptide motifs during PHF aggregation. We also show that two of the tau mutations found in hereditary frontotemporal dementias, ΔK280 and P301L, have a much stronger tendency for PHF aggregation which correlates with their high propensity for β-structure around the hexapeptide motifs.
ID 6896
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=6896
von Bergen, M., Li, L., Barghorn, S., Biernat, J., Mandelkow, E.-M., Mandelkow, E. (2001):
Mutations of tau protein in frontotemporal dementia (FTDP-17) promote aggregation of paired helical filaments by enhancing local β-structure
Mol. Biol. Cell 12 (Suppl. S), 308A