Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1177/026119290102900321
Title (Primary) Metabolic activity in primary cultures of fish hepatocytes
Author Segner, H.; Cravedi, J.
Source Titel ATLA-Alternatives to Laboratory Animals
Year 2001
Department OEC; COE
Volume 29
Issue 3
Page From 251
Page To 257
Language englisch
Keywords hepatocytes; teleost fish; biotransformation; in vitro culture; cytochrome P4501A
Abstract In aquatic toxicology, isolated liver cells from fish can be used as a tool to generate initial information on the hepatic metabolism of xenobiotics, and on the mechanisms of xenobiotic activation or deactivation. This isolation of teleost liver cells is achieved by enzymic dissociation, and monolayer cultures of fish hepatocytes in serum-free medium maintain good viability for 3-8 days. During in vitro culture, fish liver cells express stable levels of phase I and phase II enzymes, such as cytochrome P4501A or glutathione S-transferase, and the cells show an induction of biotransformation enzymes after exposure to xenobiotics. The xenobiotic metabolite pattern produced by fish hepatocytes in vitro is generally similar to that observed in vivo. Limitations to more-intensive application of cultured fish hepatocytes as a screen in aquatic hazard assessment are partly due to the rather limited scope of existing studies, i.e. the focus on one particular species (rainbow trout), and on one particular biotransformation enzyme (cytochrome P4501A), as well as a lack of comparative in vitro/in vivo studies.
Persistent UFZ Identifier
Segner, H., Cravedi, J. (2001):
Metabolic activity in primary cultures of fish hepatocytes
ATLA-Altern. Lab. Anim. 29 (3), 251 - 257 10.1177/026119290102900321