Publication Details

Reference Category Journals
DOI / URL link
Title (Primary) In situ derivatisation using pressurized liquid extraction to determine phenols, sterols, and carboxylic acids in enviromental samples and microbial biomasses
Author Pörschmann, J.; Plugge, J.; Toth, R.;
Journal Journal of Chromatography A
Year 2001
Department TUCHEM; SAN;
Volume 909
Issue 1
Language englisch;
Keywords Pressurized liquid extraction; Extraction methods; Derivatisation, GC; Peat; Soil; Environmental analysis
Abstract Pressurized liquid extraction was combined with in-situ derivatisation to extract polar analytes such as phenols (including chlorophenols) sterols and carboxylic acids from environmental and microbial samples. This one-step protocol uses acetic anhydride as an acetylation agent, N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) as an silylation agent, and boron trifluoride–methanol, phenyltrimethyl ammoniumhydroxide and trimethyl sulfoniumhydroxide as methylation agents. It results in faster extraction rates and better or comparable extraction efficiencies when compared to classical approaches. The addition of a silylation agent also facilitates the extraction kinetics of analytes not accessible to silylation (e.g. polycyclic aromatic hydrocarbons or alkylbenzenes). This may be attributed to a dissociative action of the agent to weaken analyte–matrix interactions.
ID 6685
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=6685
Pörschmann, J., Plugge, J., Toth, R. (2001):
In situ derivatisation using pressurized liquid extraction to determine phenols, sterols, and carboxylic acids in enviromental samples and microbial biomasses
J. Chromatogr. A 909 (1), 95 - 109