Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1111/j.1755-0998.2009.02574.x |
| Title (Primary) | A combination of techniques proves useful in the development of nuclear markers in the newt genus Triturus |
| Author | Themudo, G.E.; Babik, W.; Arntzen, J.W. |
| Source Titel | Molecular Ecology Resources |
| Year | 2009 |
| Department | BZF |
| Volume | 9 |
| Issue | 3 |
| Page From | 1060 |
| Page To | 1162 |
| Language | englisch |
| Abstract | To increase the number of markers available for study of phylogeny and phylogeography in the newt genus Triturus, we developed and tested 59 primer pairs using three different techniques. Primers were obtained from published sources, by designing exon-primed intron-crossing primers and from randomly cloned anonymous nuclear DNA fragments. Successful polymerase chain reaction products were cloned and sequenced. Five fragments were successfully amplified and sequenced for six species of Triturus: intron 7 of the beta-fibrinogen gene (beta fibint7), third intron of the calreticulin gene (CalintC), the 11th intron of the alpha-subunit of the platelet derived growth factor receptor (PDGFR alpha) and two anonymous markers (Cri1 and Cri4). The average percentage species divergence across all the markers is low (c. 3%), compared to what has been found in mitochondrial DNA (25-30%) |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=659 |
| Themudo, G.E., Babik, W., Arntzen, J.W. (2009): A combination of techniques proves useful in the development of nuclear markers in the newt genus Triturus Mol. Ecol. Resour. 9 (3), 1060 - 1162 10.1111/j.1755-0998.2009.02574.x |
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