Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1007/s002530100808 |
| Document | Shareable Link |
| Title (Primary) | Regiospecific effect of 1-octanol on cis-trans isomerisation of unsaturated fatty acids in the solvent-tolerant strain Pseudomonas putida S12 |
| Author | Heipieper, H.J.
; de Waard, P.; van der Meer, P.; Killian, J.A.; Isken, S.; de Bont, J.A.M.; Eggink, G.; de Wolf, F.A. |
| Source Titel | Applied Microbiology and Biotechnology |
| Year | 2001 |
| Department | UBT; SAN |
| Volume | 57 |
| Issue | 4 |
| Page From | 541 |
| Page To | 547 |
| Language | englisch |
| Abstract | The solvent-tolerant bacterium Pseudomonas putida S12, which adapts its membrane lipids to the presence of toxic solvents by a cis to trans isomerization of unsaturated fatty acids, was used to study possible in vivo regiospecificity of the isomerase. Cells were supplemented with linoleic acid (C18:2Δ9-cis,Δ12-cis), a fatty acid that cannot be synthesized by this bacterium, but which was incorporated into membrane lipids up to an amount of 15% of total fatty acids. After addition of 1-octanol, which was used as an activator of the cis-trans isomerase, the linoleic acid was converted into the Δ9-trans,Δ12-cis isomer, while the Δ9-cis,Δ12-trans and Δ9-trans,Δ12-trans isomers were not synthesized. Thus, for the first time, regiospecific in vivo formation of novel, mixed cis/trans isomers of dienoic fatty acid chains was observed. The maximal conversion (27–36% of the chains) was obtained at 0.03–0.04% (v/v) octanol, after 2 h. The observed regiospecificity of the enzyme, which is located in the periplasmic space, could be due to penetration of the enzyme to a specific depth in the membrane as well as to specific molecular recognition of the substrate molecules. |
| Heipieper, H.J., de Waard, P., van der Meer, P., Killian, J.A., Isken, S., de Bont, J.A.M., Eggink, G., de Wolf, F.A. (2001): Regiospecific effect of 1-octanol on cis-trans isomerisation of unsaturated fatty acids in the solvent-tolerant strain Pseudomonas putida S12 Appl. Microbiol. Biotechnol. 57 (4), 541 - 547 10.1007/s002530100808 |
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