Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.mycres.2009.05.002
Title (Primary) Fungi from the roots of the common terrestrial orchid Gymnadenia conopsea
Author Stark, C.; Babik, W.; Durka, W. ORCID logo
Source Titel Mycological Research
Year 2009
Department BZF
Volume 113
Issue 9
Page From 952
Page To 959
Language englisch
Keywords Fungi; Gymnadenia conopsea; ITS primers; Mycorrhiza; Orchids
Abstract The fungal community associated with the terrestrial photosynthetic orchid Gymnadenia conopsea was characterized through PCR-amplification directly from root extracted DNA and cloning of the PCR products. Six populations in two geographically distinct regions in Germany were investigated. New ITS-primers amplifying a wide taxonomic range including Basidiomycetes and Ascomycetes revealed a high taxonomic and ecological diversity of fungal associates, including typical orchid mycorrhizas of the Tulasnellaceae and Ceratobasidiaceae as well as several ectomycorrhizal taxa of the Pezizales. The wide spectrum of potential mycorrhizal partners may contribute to this orchid's ability to colonize different habitat types with their characteristic microbial communities. The fungal community of G. conopsea showed a clear spatial structure. With 43 % shared taxa the species composition of the two regions showed only little overlap. Regardless of regions, populations were highly variable concerning taxon richness, varying between 5 and 14 taxa per population. The spatial structure and the continuous presence of mycorrhizal taxa on the one hand and the low specificity towards certain fungal taxa on the other hand suggest that the fungal community associated with G. conopsea is determined by multiple factors. In this context, germination as well as pronounced morphological and genetic differentiation within G. conopsea deserve attention as potential factors affecting the composition of the fungal community.
Persistent UFZ Identifier
Stark, C., Babik, W., Durka, W. (2009):
Fungi from the roots of the common terrestrial orchid Gymnadenia conopsea
Mycol. Res. 113 (9), 952 - 959 10.1016/j.mycres.2009.05.002