Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1002/etc.5620200324
Document Shareable Link
Title (Primary) Polycyclic aromatic hydrocarbons as inducers of cytochrome P4501A enzyme activity in the rainbow trout liver cell line, RTL-W1, and primary cultures of rainbow trout hepatocytes
Author Behrens, A.; Schirmer, K.; Bols, N.C.; Segner, H.
Source Titel Environmental Toxicology and Chemistry
Year 2001
Department OEC; COE
Volume 20
Issue 3
Page From 632
Page To 643
Language englisch
Keywords Polycyclic aromatic hydrocarbons; Rainbow trout; 7-Ethoxyresorufin-O-deethylase; RTL-W1 cell line; Intact primary hepatocytes
Abstract In order to investigate cell-specific differences in the response of in vitro models to environmental toxicants, we compared the capacity of nine polycyclic aromatic hydrocarbons (PAHs) to induce cytochrome P4501A (CYP1A) in primary rainbow trout (Oncorhynchus mykiss) hepatocytes and a rainbow trout liver cell line, RTL-W1. Induction of CYP1A was estimated from the catalytic activity of 7-ethoxyresorufin-O-deethylase (EROD) and compared by median effective concentration (EC50) values, induction spans, and benzo[a]pyrene induction equivalency factors for inducing PAHs. The influence of culture conditions was investigated with respect to the presence or absence of serum and varying exposure times. Both in vitro systems lead to an identical classification of the PAHs in noninducing (anthracene, fluoranthene, phenanthrene, and pyrene) and inducing compounds with a similar ranking of inducing PAHs. Mean EC50 values in RTL-W1 cells were, respectively, 343 and 266 nM for benzo[a]anthracene, 57 and 92 nM for BaP, 134 and 283 nM for benzo[b]fluoranthene, 455 and 270 nM for chrysene, and 98 and 116 nM for 3-methylcholanthrene. Compared to primary hepatocytes, the RTL-W1 cell line was more sensitive in its EROD response to the presence or absence of serum and to the increase in exposure time, which led to higher EC50 values.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=6197
Behrens, A., Schirmer, K., Bols, N.C., Segner, H. (2001):
Polycyclic aromatic hydrocarbons as inducers of cytochrome P4501A enzyme activity in the rainbow trout liver cell line, RTL-W1, and primary cultures of rainbow trout hepatocytes
Environ. Toxicol. Chem. 20 (3), 632 - 643 10.1002/etc.5620200324