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Title (Primary) The two enantiospecific dichlorprop/α-ketoglutarate-dioxygenases from Delftia acidovorans MC1 - protein and sequence data of RdpA and SdpA
Author Westendorf, A.; Benndorf, D.; Müller, R.H.; Babel, W.;
Journal Microbiological Research
Year 2002
Department UMB; PROTEOM;
Volume 157
Issue 4
Language englisch;
Keywords chiral herbicides; (S)-phenoxypropionate/α-ketoglutarate-dioxygenase (SdpA); (R)-phenoxypropionate/α-ketoglutarate-dioxygenase (RdpA); partial amino acid sequences; Delftia acidovorans MC1
Abstract Two a-ketoglutarate-dependent dioxygenases carrying enantiospecific activity for the etherolytic cleavage of racemic phenoxypropionate herbicides [(RS)-2-(2,4-dichlorophenoxy)propionate and (RS)-2-(4-chloro-2-methylphenoxy)propionate] from Delftia acidovorans MC1 were characterized with respect to protein and sequence data. The (S)-phenoxypropionate/a-ketoglutarate-dioxygenase (SdpA) appeared as a monomeric enzyme with a molecular weight of 32 kDa in the presence of SDS. N-terminal sequences revealed relationship to a-ketoglutarate-dependent taurine dioxygenase (TauD) and to 2,4-dichlorophenoxyacetate/a-ketoglutarate-dioxygenase (TfdA). The (R)-phenoxypropionate/a-ketoglutarate-dioxygenase (RdpA) referred to 36 kDa in the presence of SDS and to 108 kDa under native conditions. Internal sequences of fragments obtained after digestion made evident relationship to TfdA and TauD. Two-dimensional electrophoretic separation resulted in the resolution of up to 3 individual spots with almost identical molecular weights but different isoelectric points with both RdpA and SdpA. The structural differences of these isoenzyme forms are not yet clear.
ID 6137
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=6137
Westendorf, A., Benndorf, D., Müller, R.H., Babel, W. (2002):
The two enantiospecific dichlorprop/α-ketoglutarate-dioxygenases from Delftia acidovorans MC1 - protein and sequence data of RdpA and SdpA
Microbiol. Res. 157 (4), 317 - 322