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Title (Primary) Quantification of polymerisation processes during the oxidative degradation of 14C-labelled chlorophenols
Author Geyer, R.; Richnow, H.H.; Schlosser, D.;
Journal Water Air and Soil Pollution: Focus
Year 2002
Department ISOBIO; UMB;
Volume 2
Issue 3
Language englisch;
Abstract In experiments employing the lignocellulose-decaying basidiomycetes Trametes versicolor and Stropharia rugosoannulata degrading uniformly14C-labelled 2,4-dichlorophenol and pentachlorophenol, acombination of size exclusion chromatography (SEC),fractionation, and β-scintillation counting wasapplied to quantify polymerisation products formed duringchlorophenol degradation. Time-dependent mass balances weregenerated by analysis of 14C in polymerisation products,CO2, as well as monomer non-polar and polar metabolites.Approximately 30% of the chlorophenols were found to bepolymerised. A major fraction of the polymerised productscorresponded to a molecular weight range from 0.24 – 40 kDa.Only a minor fraction could be attributed to a molecularweight >40 kDa. This method proved to be useful inquantification of polymerisation products and kinetics of thepolymerisation processes, whereas UV/Vis detection ofpolymerisation products separated by SEC led to false positiveresults. The SEC-14C method could also be applied forother complex processes where polymerisation ordepolymerisation occurs (humification, degradation oflignocellulose, formation of bound residues from xenobioticssuch as polycyclic aromatic hydrocarbons or 2,4,6-trinitrotoluene) and where spectrophotometric determinationsare difficult or impossible.
ID 5510
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=5510
Geyer, R., Richnow, H.H., Schlosser, D. (2002):
Quantification of polymerisation processes during the oxidative degradation of 14C-labelled chlorophenols
Water Air Soil Poll.: Focus 2 (3), 153 - 159