Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1078/0176-1617-00525
Title (Primary) Heat shock protein synthesis is induced by diethyl phthalate but not by di(2-ethylhexyl) phthalate in radish (Raphanus sativus)
Author Saarma, K.; Tarkka, M.T.; Itavaara, M.; Fagerstedt, K.V.
Source Titel Journal of Plant Physiology
Year 2003
Department BOOEK
Volume 160
Issue 9
Page From 1001
Page To 1010
Language englisch
Keywords diethyl phthalate; di(2-ethylhexyl) phthalate; heat shock proteins; Raphanus sativus; stress proteins
Abstract The toxicity and effects on protein synthesis of the phthalate esters diethyl phthalate (DEP) and di(2-ethylhexyl) phthalate (DEHP) was studied in radish seedlings (Raphanus sativus cv. Koopenhaminan tori). Phthalate esters are a class of commercially important compounds used mainly as plasticizers in high molecular-weight polymers such as many plastics. They can enter soil through various routes and can affect plant growth and development, First the effect of DEP and DEHP on the growth of radish seedlings was determined in an aqueous medium. It was found that DEP, but not DEHP, caused retardation of growth in radish. A further investigation on protein synthesis during DEP-stress was executed by in vivo protein labeling combined with two-dimensional gel electrophoresis (2D-PAGE). For comparisons with known stress-induced proteins a similar experiment was done with heat shock, and the induced heat shock proteins (HSPs) were compared with those of DEP-stress. The results showed that certain HSPs can be used as an indicator of DEP-stress, although the synthesis of most HSPs was not affected by DEP. DEP also elicited the synthesis of numerous proteins found only in DEP-treated roots. The toxic effect of phthalate esters and the roles of the induced proteins are discussed.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=5173
Saarma, K., Tarkka, M.T., Itavaara, M., Fagerstedt, K.V. (2003):
Heat shock protein synthesis is induced by diethyl phthalate but not by di(2-ethylhexyl) phthalate in radish (Raphanus sativus)
J. Plant Physiol. 160 (9), 1001 - 1010 10.1078/0176-1617-00525