Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1007/s00203-003-0522-y |
| Document | Shareable Link |
| Title (Primary) | Biodegradation of bis(1-chloro-2-propyl) ether via initial ether scission and subsequent dehalogenation by Rhodococcus sp. strain DTB |
| Author | Moreno Horn, M.; Garbe, L.-A.; Tressl, R.; Adrian, L.
; Görisch, H. |
| Source Titel | Archives of Microbiology |
| Year | 2003 |
| Department | ISOBIO |
| Volume | 179 |
| Issue | 4 |
| Page From | 234 |
| Page To | 241 |
| Language | englisch |
| Abstract | Rhodococcus sp. strain DTB (DSM 44534) grows on bis(1-chloro-2-propyl) ether (DDE) as sole source of carbon and energy. The non-chlorinated diisopropyl ether and bis(1-hydroxy-2-propyl) ether, however, did not serve as substrates. In ether degradation experiments with dense cell suspensions, 1-chloro-2-propanol and chloroacetone were formed, which indicated that scission of the ether bond is the first step while dehalogenation of the chlorinated C3-compounds occurs at a later stage of the degradation pathway. Inhibition of ether scission by methimazole suggested that the first step in degradation is catalyzed by a flavin-dependent enzyme activity. The non-chlorinated compounds 1,2-propanediol, hydroxyacetone, lactate, pyruvate, 1-propanol, propanal, and propionate also supported growth, which suggested that the intermediates 1,2-propanediol and hydroxyacetone are converted to pyruvate or to propionate, which can be channeled into the citric acid cycle by a number of routes. Total release of chloride and growth-yield experiments with DDE and non-chlorinated C3-compounds suggested complete biodegradation of the chlorinated ether. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=5074 |
| Moreno Horn, M., Garbe, L.-A., Tressl, R., Adrian, L., Görisch, H. (2003): Biodegradation of bis(1-chloro-2-propyl) ether via initial ether scission and subsequent dehalogenation by Rhodococcus sp. strain DTB Arch. Microbiol. 179 (4), 234 - 241 10.1007/s00203-003-0522-y |
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