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Title (Primary) Sequential fractionation procedure for the identification of potentially cytochrome P4501A-inducing compounds
Author Brack, W.; Kind, T.; Hollert, H.; Schrader, S.; Möder, M.;
Journal Journal of Chromatography A
Year 2003
Department WANA; ANA; COE;
Volume 986
Issue 1
Language englisch;
Keywords Multistep fractionation; Preparative chromatography
Abstract A multistep fractionation procedure for the separation of nonpolar aromatic compounds with respect to cytochrome P4501A induction is presented. Normal-phase HPLC on nitrophenylpropyl silica and cyanopropyl silica was tested for group-specific separation as a first fractionation step. Subsequent individual compound-specific PAH fractionation was done by means of reversed-phase HPLC. Electron-donor–acceptor HPLC and size-exclusion chromatography were applied to separate PAHs, PCBs, PCNs and PCDD/Fs according to their number of aromatic carbon atoms, their hydrophobicity, their degree of chlorination, their planarity and their molecular size. The method was validated for complex environmental mixtures on the basis of two sediment extracts.
ID 4741
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=4741
Brack, W., Kind, T., Hollert, H., Schrader, S., Möder, M. (2003):
Sequential fractionation procedure for the identification of potentially cytochrome P4501A-inducing compounds
J. Chromatogr. A 986 (1), 55 - 66