Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1897/03-414 |
| Document | Shareable Link |
| Title (Primary) | Fate and metabolism of [15N]2,4,6-trinitrotoluene in soil |
| Author | Weiß, M.; Geyer, R.; Russow, R.; Richnow, H.H.; Kästner, M. |
| Source Titel | Environmental Toxicology and Chemistry |
| Year | 2004 |
| Department | ISOBIO; UMB; UBT; BOPHY |
| Volume | 23 |
| Issue | 8 |
| Page From | 1852 |
| Page To | 1860 |
| Language | englisch |
| Keywords | [15N]2,4,6-Trinitrotoluene; Biodegradation; Fungi; Azoxytetranitrotoluene; Dinitrogen oxide |
| Abstract | The fates of the labels from [14C] and [15N] trinitrotoluene were analyzed in bioreactors under aerobic conditions in soil treated by a fungal bioremediation process with Stropharia rugosoannulata and in control soil. Up to 17.5% of the 15N label had a different fate than the 14C label. Three N-mineralization processes were identified in detailed experiments with [15N]TNT. About 2% of the 15N label was found as NO3− and NH4−, showing simultaneous processes of direct TNT denitration (I) and reduction with cleavage of the amino groups (II). The enrichment of NO2−/NO3− (up to 7.5 atom% 15N abundance) indicates the formation of Meisenheimer complexes with a denitration of [15N]TNT. A 1.4% of the label was found distributed between N2O and N2. However, the 15N enrichment of the N2O (up to 38 atom%) demonstrated that both N atoms were generated from the labeled TNT and clearly indicates a novel formation process (III). We propose, as an explanation, the generation of N2O by cleavage from condensed azoxy metabolites. In addition, 1.7% of the 15N label was detected as biogenic amino acids in the wheat straw containing the fungus. Overall, 60 to 85% of the applied [15N]TNT was degraded and 52 to 64% was found as nonextractable residues in the soil matrix. Three percent was detected as 2-amino-4,6-dinitrotoluene and 4-amino-2,6-dinitrotoluene. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=4634 |
| Weiß, M., Geyer, R., Russow, R., Richnow, H.H., Kästner, M. (2004): Fate and metabolism of [15N]2,4,6-trinitrotoluene in soil Environ. Toxicol. Chem. 23 (8), 1852 - 1860 10.1897/03-414 |
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