Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/s0169-7722(03)00092-5 |
| Title (Primary) | Bioremediation of chlorobenzene-contaminated ground water in an in situ reactor mediated by hydrogen peroxide |
| Author | Vogt, C.; Alfreider, A.; Lorbeer, H.; Hoffmann, D.; Wuensche, L.; Babel, W. |
| Source Titel | Journal of Contaminant Hydrology |
| Year | 2004 |
| Department | ISOBIO; UMB |
| Volume | 68 |
| Issue | 1 |
| Page From | 121 |
| Page To | 141 |
| Language | englisch |
| Keywords | Aquifer; Chlorobenzene; Enhanced natural attenuation; Degradation; Hydrogen peroxide; Nitrate reduction |
| Abstract | New in situ reactive barrier technologies were tested nearby a local aquifer in Bitterfeld, Saxonia-Anhalt, Germany, which is polluted mainly by chlorobenzene (CB), in concentrations up to 450 μM. A reactor filled with original aquifer sediment was designed for the microbiological remediation of the ground water by indigenous bacterial communities. Two remediation variants were examined: (a) the degradation of CB under anoxic conditions in the presence of nitrate; (b) the degradation of CB under mixed electron acceptor conditions (oxygen+nitrate) using hydrogen peroxide as the oxygen-releasing compound. Under anoxic conditions, no definite degradation of CB was observed. Adding hydrogen peroxide (2.94 mM) and nitrate (2 mM) led to the disappearance of CB (ca. 150 μM) in the lower part of the reactor, accompanied by a strong increase of the number of cultivable aerobic CB degrading bacteria in reactor water and sediment samples, indicating that CB was degraded mainly by productive bacterial metabolism. Several aerobic CB degrading bacteria, mostly belonging to the genera Pseudomonas and Rhodococcus, were isolated from reactor water and sediments. In laboratory experiments with reactor water, oxygen was rapidly released by hydrogen peroxide, whereas biotic-induced decomposition reactions of hydrogen peroxide were almost four times faster than abiotic-induced decomposition reactions. A clear chemical degradation of CB mediated by hydrogen peroxide was not observed. CB was also completely degraded in the reactor after reducing the hydrogen peroxide concentration to 880 μM. The CB degradation completely collapsed after reducing the hydrogen peroxide concentration to 440 μM. In the following, the hydrogen peroxide concentrations were increased again (to 880 μM, 2.94 mM, and 880 μM, respectively), but the oxygen demand for CB degradation was higher than observed before, indicating a shift in the bacterial population. During the whole experiment, nitrate was uniformly reduced during the flow path in the reactor. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=4610 |
| Vogt, C., Alfreider, A., Lorbeer, H., Hoffmann, D., Wuensche, L., Babel, W. (2004): Bioremediation of chlorobenzene-contaminated ground water in an in situ reactor mediated by hydrogen peroxide J. Contam. Hydrol. 68 (1), 121 - 141 10.1016/s0169-7722(03)00092-5 |
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