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Title (Primary) The single-nucleotide primer extension (SNuPE) method for the multiplex detection of various DNA sequences: from detection of point mutations to microbial ecology
Author Nikolausz, M.; Chatzinotas, A.; Táncsics, A.; Imfeld, G.; Kästner, M.;
Journal Biochemical Society Transactions
Year 2009
Department ISOBIO; UMB; UBT; BIOENERGIE;
Volume 37
Issue 2
Language englisch;
Keywords microbiology; minisequencing; multiplex screening; mutation detection; single-nucleotide polymorphism; single-nucleotide primer extension (SNuPE)
Abstract Methods based on SNuPE (single-nucleotide primer extension) have become invaluable tools for the rapid and highly specific detection of point mutations and single-nucleotide polymorphisms in the field of human genetics. In the primer extension reaction, a DNA polymerase is used to label a specific primer hybridized to the target sequence by incorporating a single labelled ddNTP (dideoxynucleotide). This labelling provides not only information about the complementary nucleotide of interest in the opposite strand but also a semiquantitative analysis of the sequence targeted by the primer. Since several subdisciplines of microbiology increasingly require cultivation-independent molecular screening tools for elucidating differences between either strains or community structures based on sequence variations of marker genes, SNuPE offers a promising alternative to the existing tool box. The present review describes the method in detail and reports the state-of-the-art applications of this technique both in the field of nucleic acid detections in human genetics and in microbiology.
ID 447
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=447
Nikolausz, M., Chatzinotas, A., Táncsics, A., Imfeld, G., Kästner, M. (2009):
The single-nucleotide primer extension (SNuPE) method for the multiplex detection of various DNA sequences: from detection of point mutations to microbial ecology
Biochem. Soc. Trans. 37 (2), 454 - 459