Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.freeradbiomed.2004.04.003 |
| Title (Primary) | Involvement of lipid peroxidation and organic peroxides in UVA-induced matrix metalloproteinase-1 expression |
| Author | Polte, T.; Tyrrell, R.M. |
| Source Titel | Free Radical Biology and Medicine |
| Year | 2004 |
| Department | IMMU |
| Volume | 36 |
| Issue | 12 |
| Page From | 1566 |
| Page To | 1574 |
| Language | englisch |
| Keywords | UVA; Collagenase; Lipid peroxidation; Skin fibroblasts; Free radicals |
| Abstract | Ultraviolet A (UVA) irradiation causes human skin aging and skin cancer at least partially through the activation of matrix metalloproteinases (MMPs). MMP-1, the interstitial collagenase, is responsible for the degradation of collagen and is involved in tumor progression in human skin. The present study uses human skin fibroblast cells (FEK4) to investigate the involvement of lipid peroxidation and the role of peroxides as possible mediators in MMP-1 activation by UVA. Preincubation with the antioxidants butylated hydroxytoluene and Trolox reduced UVA-dependent MMP-1 upregulation, suggesting that peroxidation of membrane lipids is involved. Blocking the iron-driven generation of lipid peroxides and hydroxyl radicals by different iron chelators led to a decrease in UVA-induced MMP-1 mRNA accumulation. Moreover, modulation of glutathione peroxidase activity by use of the specific inhibitor mercaptosuccinate (MS) or by the depletion of glutathione (using buthionine-S, R-sulfoximine, BSO), enhanced the UVA-dependent MMP-1 response. Finally, UVA irradiation generated a significant increase in intracellular peroxide levels which is augmented by pretreatment of the cells with BSO or MS. Our results demonstrate that lipid peroxidation and the production of peroxides are important events in the signalling pathway of MMP-1 activation by UVA. (C) 2004 Elsevier Inc. All rights reserved |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=4436 |
| Polte, T., Tyrrell, R.M. (2004): Involvement of lipid peroxidation and organic peroxides in UVA-induced matrix metalloproteinase-1 expression Free Radic. Biol. Med. 36 (12), 1566 - 1574 10.1016/j.freeradbiomed.2004.04.003 |
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