Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1264/jsme2.19.1 |
| Title (Primary) | Two-photon imaging for studying the microbial ecology of biofilm systems |
| Author | Neu, T.R.; Walczysko, P.; Lawrence, J.R. |
| Journal | Microbes and Environments |
| Year | 2004 |
| Department | FLOEK |
| Volume | 19 |
| Issue | 1 |
| Page From | 1 |
| Page To | 6 |
| Language | englisch |
| Abstract | Conventional confocal laser scanning microscopy uses continuous wave visible or UV lasers for one-photon excitation and imaging. These instruments are now enhanced with pulsed infrared lasers allowing two-photon excitation. With the appropriate technical setup two-photon excitation may be used for intensity imaging or for lifetime imaging. Both of these techniques have specific advantages if compared with one-photon excitation. Despite this fact, to date mainly one-photon excitation has been employed in microbial ecology. In this review the potential of two-photon intensity and lifetime imaging is discussed, using appropriate examples from cell biology as well as the first reports in microbiology. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=4394 |
| Neu, T.R., Walczysko, P., Lawrence, J.R. (2004): Two-photon imaging for studying the microbial ecology of biofilm systems Microbes Environ. 19 (1), 1 - 6 |
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