Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.soilbio.2004.02.017
Title (Primary) Diversity of laccase genes from basidiomycetes in a forest soil
Author Luis, P.; Walther, G.; Kellner, H.; Martin, F.; Buscot, F.
Source Titel Soil Biology & Biochemistry
Year 2004
Department BOOEK
Volume 36
Issue 7
Page From 1025
Page To 1036
Language englisch

Fungal oxidative exo-enzymes lacking substrate specificity play a central role in the cycling of soil organic matter. Due to their broad ecological impact and available knowledge of their gene structure, laccases appeared to be appropriate markers to monitor fungi with this kind of oxidative potential in soils. A degenerate PCR-primer pair Cu1F/Cu2R, specific for basidiomycetes, was designed to assess directly the diversity of laccase genes in soils. PCR amplification of mycelial cultures and fruit-bodies of a wide spectrum of basidiomycetes, covering all functional groups (saprophytes, symbionts, and pathogens), produced multiple DNA fragments around 200 bp. A neighbor-joining tree analysis of the PCR-amplified laccase sequences showed a clear species-specificity, but also revealed that most fungal taxa possess several laccase genes showing a large sequence divergence. This sequence diversity precluded the systematic attribution of amplified laccase of unknown origin to specific taxa. Amplification of laccase sequences from DNA, extracted from a brown (moder) forest soil, showed a specific distribution of laccase genes and of the corresponding fungal species in the various soil horizons (O-h, A(h), B-v) The most organic O-h-horizon displayed the highest gene diversity. Saprophytic fungi appeared to be less widespread through the soil horizons and displayed a higher diversity of laccase genes than the mycorrhizal ones.
Persistent UFZ Identifier
Luis, P., Walther, G., Kellner, H., Martin, F., Buscot, F. (2004):
Diversity of laccase genes from basidiomycetes in a forest soil
Soil Biol. Biochem. 36 (7), 1025 - 1036 10.1016/j.soilbio.2004.02.017