Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1007/s00253-004-1734-z |
| Document | Shareable Link |
| Title (Primary) | Carbon isotope fractionation during cis-trans isomerization of unsaturated fatty acids in Pseudomonas putida |
| Author | Heipieper, H.J.
; Neumann, G.; Kabelitz, N.; Kästner, M.; Richnow, H.H. |
| Source Titel | Applied Microbiology and Biotechnology |
| Year | 2004 |
| Department | ISOBIO; UBT |
| Volume | 66 |
| Issue | 3 |
| Page From | 285 |
| Page To | 290 |
| Language | englisch |
| Abstract | The molecular mechanism of the unique cis to trans isomerization of unsaturated fatty acids in the solvent-tolerant bacterium Pseudomonas putida S12 was studied. For this purpose, the carbon isotope fractionation of the cis–trans isomerase was estimated. In resting cell experiments, addition of 3-nitrotoluene for activation of the cis–trans isomerase resulted in the conversion of the cis-unsaturated fatty acids into the corresponding trans isomers. For the conversion of C16:1 cis to its corresponding trans isomer, a significant fractionation was measured. The intensity of this fractionation strongly depended on the rate of cis–trans isomerization and the added concentration of 3-nitrotoluene, respectively. The presence of a significant fractionation provides additional indication for a transition from the sp2 carbon linkage of the cis-double bond to an intermediate sp3 within an enzyme–substrate complex. The sp2 linkage is reconstituted after rotation to the trans configuration has occurred. As cytochrome c plays a major role in the catabolism of Cti polypeptide, these findings favour a mechanism for the enzyme in which electrophilic iron (Fe3+), provided by a heme domain, removes an electron of the cis double bond thereby transferring the sp2 linkage into sp3. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=4169 |
| Heipieper, H.J., Neumann, G., Kabelitz, N., Kästner, M., Richnow, H.H. (2004): Carbon isotope fractionation during cis-trans isomerization of unsaturated fatty acids in Pseudomonas putida Appl. Microbiol. Biotechnol. 66 (3), 285 - 290 10.1007/s00253-004-1734-z |
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