| Publication Details | 
| Category | Text Publication | 
| Reference Category | Journals | 
| DOI | 10.1007/s00253-004-1693-4 | 
| Document | Shareable Link | 
| Title (Primary) | Degradation of estradiol and ethinyl estradiol by activated sludge and by a defined mixed culture | 
| Author | Weber, S.; Leuschner, P.; Kämpfer, P.; Dott, W.; Hollender, J. | 
| Source Titel | Applied Microbiology and Biotechnology | 
| Year | 2005 | 
| Department | ISOBIO | 
| Volume | 67 | 
| Issue | 1 | 
| Page From | 106 | 
| Page To | 112 | 
| Language | englisch | 
| Abstract | The aerobic degradation of the natural hormone 17-β-estradiol (E2) and the synthetic hormone 17-α-ethinyl estradiol (EE2) was investigated in batch experiments with activated sludge from a conventional and a membrane sewage treatment plant. E2 was converted to estrone (E1), the well known metabolite, and further completely transformed within 3 days. The turnover rates of E2 did not differ greatly between conventional and membrane activated sludge. EE2 was persistent in both sludges. By several transfers into fresh E2-medium an enrichment culture could be selected that used E2 as growth substrate. Further enrichment and isolation led to a defined mixed culture consisting of two strains, which were identified by a polyphasic approach as Achromobacter xylosoxidans and Ralstonia sp., respectively. The culture used E2 and E1 as growth substrates and transformed estriol (E3) and 16-α-hydroxyestrone but not the xenoestrogens bisphenol A, α-zearalenol, mestranol or EE2. The turnover rates of E2 were 0.025–0.1 μg h−1 cfu−1 and did not depend on the steroid concentration. | 
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=3883 | 
| Weber, S., Leuschner, P., Kämpfer, P., Dott, W., Hollender, J. (2005): Degradation of estradiol and ethinyl estradiol by activated sludge and by a defined mixed culture Appl. Microbiol. Biotechnol. 67 (1), 106 - 112 10.1007/s00253-004-1693-4 | |
