Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.bbrc.2005.02.158
Title (Primary) Yeast adaptation to 2,4-dichlorophenoxyacetic acid involves increased membrane fatty acid saturation degree and decreased OLE1 transcription
Author Viegas, C.A.; Cabral, M.G.; Teixeira, M.C.; Neumann, G.; Heipieper, H.J. ORCID logo ; Sá-Correia, I.
Source Titel Biochemical and Biophysical Research Communications
Year 2005
Department UBT
Volume 330
Issue 1
Page From 271
Page To 278
Language englisch
Keywords Saccharomyces cerevisiae; 2,4-D; Fatty acid unsaturation degree; Plasma membrane; OLE1; Stress response; Lipophilic acids; Herbicides
Abstract Yeast cells adapted to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) exhibit a plasma membrane less susceptible to 2,4-D-induced disruption and are more tolerant than unadapted cells to lethal concentrations of the herbicide. These cells, adapted to grow in the presence of increasing concentrations of 2,4-D, were found to exhibit a dose-dependent increase of the saturation degree of membrane fatty acids, associated to the higher percentage of stearic (C18:0) and palmitic (C16:0) acids, and to the decreased percentage of palmitoleic (D9-cisC16:1) and oleic (D9-cisC18:1) acids. The decreased transcription of the OLE1 gene (encoding the D9 fatty acid desaturase that catalyses the conversion of palmitic and stearic acids to palmitoleic and oleic acids, respectively) registered in 2,4-D adapted cells suggests that yeast adaptation to the herbicide involves the enhancement of the ratio of saturated (C16:0 and C18:0) to monounsaturated (C16:1 and C18:1) membrane fatty acids through a reduced OLE1 expression.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=3862
Viegas, C.A., Cabral, M.G., Teixeira, M.C., Neumann, G., Heipieper, H.J., Sá-Correia, I. (2005):
Yeast adaptation to 2,4-dichlorophenoxyacetic acid involves increased membrane fatty acid saturation degree and decreased OLE1 transcription
Biochem. Biophys. Res. Commun. 330 (1), 271 - 278 10.1016/j.bbrc.2005.02.158