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Title (Primary) Observation of bias associated with re-amplification of DNA isolated from denaturing gradient gels
Author Nikolausz, M.; Sipos, R.; Révész, S.; Székely, A.; Márialigeti, K.;
Journal FEMS Microbiology Letters
Year 2005
Department UBT;
Volume 244
Issue 2
Language englisch;
Keywords 16S rDNA; DGGE; PCR; Bias; Re-amplification
Abstract DNA from environmental PCR products separated by denaturing gradient gel electrophoresis (DGGE) was isolated from the background smear rather than from discrete bands of the DGGE gel. The “interband” region was considered as a potential source of less dominant members of natural microbial communities. Surprisingly, instead of detecting new bands from the re-amplified PCR products, patterns very similar to the original ones were obtained regardless of the position of the “interband” region. The results suggest that the separation of amplicons by DGGE may not be perfect and band re-amplification based sequence analyses need careful interpretation.
ID 3655
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=3655
Nikolausz, M., Sipos, R., Révész, S., Székely, A., Márialigeti, K. (2005):
Observation of bias associated with re-amplification of DNA isolated from denaturing gradient gels
FEMS Microbiol. Lett. 244 (2), 385 - 390