Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.jtherbio.2004.06.002
Title (Primary) Combined effects of temperature and cadmium on developmental parameters and biomarker responses in zebrafish (Danio rerio) embryos
Author Hallare, A.V.; Schirling, M.; Luckenbach, T. ORCID logo ; Köhler, H.-R.; Triebskorn, R.
Source Titel Journal of Thermal Biology
Year 2005
Department ZELLTOX
Volume 30
Issue 1
Page From 7
Page To 17
Language englisch
Keywords Temperature; Cadmium; Biomarker; Liver histopathology; Heat shock proteins; Embryotoxicity; Zebrafish; Danio rerio
Abstract To determine the interactions between temperature and cadmium on zebrafish (Danio rerio) development, fertilized eggs were exposed to combinations of three temperature levels (21 °C, 26 °C, and 33 °C) and six cadmium concentrations (0, 0.25, 0.5, 2.0, 5.0, and 10.0 mg/L). Endpoints used included LC50 value (48 h), developmental rate, mortality, heart rate, hatching success, liver histopathology, embryo abnormalities, and heat shock protein (hsp) induction. Results showed a significant acceleration in the developmental rate with increasing temperature and irrespective of the presence of cadmium. Data on LC50 and ELS-test revealed that simultaneous exposure to both cadmium ions and cold stress (21 °C) was highly detrimental to growing embryos, causing a pronounced mortality and a significant reduction in average heart rate and embryo hatchability. In contrast, no similar reactions to cadmium were observed in pre-hatched embryos exposed to both control (26 °C) and high temperature (33 °C), and this can be explained by the significantly higher expression of hsp (hsp70) in embryos at these temperatures. Upon hatching, however, the larvae showed increased sensitivity to cadmium. The severity of malformations in the post-hatched larvae was in the order: hot cadmium stress>cold cadmium stress>cadmium stress alone>no stress at all. Liver histopathology as well as depletion in glycogen reserves exhibited greater severity with increasing cadmium concentration, irrespective of temperature. The present study confirms that temperature effectively confounds cadmium toxicity and needs to be considered for the accurate prediction and assessment of cadmium-induced toxicity in fish.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=3398
Hallare, A.V., Schirling, M., Luckenbach, T., Köhler, H.-R., Triebskorn, R. (2005):
Combined effects of temperature and cadmium on developmental parameters and biomarker responses in zebrafish (Danio rerio) embryos
J. Therm. Biol. 30 (1), 7 - 17 10.1016/j.jtherbio.2004.06.002