Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.scitotenv.2004.12.002
Title (Primary) Assessing contamination levels of Laguna Lake sediments (Philippines) using a contact assay with zebrafish (Danio rerio) embryos
Author Hallare, A.V.; Kosmehl, T.; Schulze, T. ORCID logo ; Hollert, H.; Köhler, H.-R.; Triebskorn, R.
Journal Science of the Total Environment
Year 2005
Department WANA
Volume 347
Issue 1-3
Page From 254
Page To 271
Language englisch
Keywords Laguna Lake; Sediment toxicity; Embryotoxicity; Heat shock proteins; Perylene; PAHs; Heavy metals; Zebrafish; Danio rerio; Bioavailability
Abstract The present study investigated the suitability of a sediment contact assay using zebrafish (Danio rerio) embryos to evaluate the degree of lake sediment contamination. As endpoints, developmental parameters (mortality, abnormality, heart rate, and hatching rate) as well as stress protein responses (hsp 70 levels) in the developing embryos were recorded during a 96-h exposure. Fertilized zebrafish eggs were exposed to both the whole as well as organic extract concentrations prepared from collected sediments from 5 sites along Laguna Lake, Philippines. Compared to whole sediment exposure, more severe embryotoxic and teratogenic responses were elicited in embryos exposed to organic extracts. However, since whole sediment-exposed embryos also revealed significant developmental defects, this exposure phase served as the more realistic exposure scenario in our study. Weak to strong upregulation of hsp 70 levels was also registered among embryos exposed to both whole sediments and organic extracts. The observed embryotoxic and proteotoxic responses by zebrafish embryos to Laguna Lake sediment exposures were discussed in relation to the analyzed contaminants in the sediments (heavy metals, PAHs (perylene), solvent). Overall, the present study points out that the sediment contact assay with zebrafish embryos offers a practicable and highly sensitive bioassay for the general assessment of sediment toxicity.
Persistent UFZ Identifier
Hallare, A.V., Kosmehl, T., Schulze, T., Hollert, H., Köhler, H.-R., Triebskorn, R. (2005):
Assessing contamination levels of Laguna Lake sediments (Philippines) using a contact assay with zebrafish (Danio rerio) embryos
Sci. Total Environ. 347 (1-3), 254 - 271