Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1007/s00210-005-1070-6 |
| Document | Shareable Link |
| Title (Primary) | Evidence for the existence of P2Y1,2,4 receptor subtypes in HEK-293 cells: reactivation of P2Y1 receptors after repetitive agonist application |
| Author | Fischer, W.; Franke, H.; Gröger-Arndt, H.; Illes, P. |
| Source Titel | Naunyn-Schmiedebergs Archives of Pharmacology |
| Year | 2005 |
| Department | PROTEOM |
| Volume | 371 |
| Issue | 6 |
| Page From | 466 |
| Page To | 472 |
| Language | englisch |
| Abstract | ATP, ADP beta S and UTP induced a comparable rise in the intracellular Ca2+ concentration ([Ca2+](i)) in HEK-293 cells using fura-2 microfluorimetry. The responses persisted in Ca2+-free medium, but were abolished following depletion of intracellular Ca2+ stores by cyclopiazonic acid. Cross-desensitisation experiments demonstrated that exposure to ADPOS has no marked effect on UTP-induced [Ca2+]i transients and vice versa. 6 Whereas the P2Y(1) receptor-selective antagonist 2'-deoxy-N-6-methyladenosine 3',5'-diphosphate (MRS 2179) abolished the responses to ADP S, it decreased and did not alter the responses to ATP and UTP respectively. Although the P2Y(1)/ P2Y(4) receptor-preferential antagonist pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS) abolished the responses to ADPOS, and decreased those to ATP, it also depressed the UTP-induced [Ca2+](i) transients. Suramin, an antagonist with preference for P2Y(2) receptors decreased both the ATP- and UTP-induced [Ca2+](i) reactions. After numerous splittings, HEK-293 cells failed to react to ADPOS; however, repeated superfusion with this P2Y(1) receptor agonist restored the [Ca2+](i) signals. In agreement with the functional data, real-time polymerase chain reaction and immunocytochemical studies indicated the presence of P2Y(1), P2Y(2) and P2Y(4) receptors. Our findings raise doubt with respect to the reliability of HEK-293 cells as expression systems for recombinant P2X receptors, because of a possible functional interaction with endogenous P2Y receptors |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=3319 |
| Fischer, W., Franke, H., Gröger-Arndt, H., Illes, P. (2005): Evidence for the existence of P2Y1,2,4 receptor subtypes in HEK-293 cells: reactivation of P2Y1 receptors after repetitive agonist application Naunyn-Schmiedebergs Arch. Pharmacol. 371 (6), 466 - 472 10.1007/s00210-005-1070-6 |
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