Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.hazadv.2026.101705 |
Licence  |
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| Title (Primary) | Single and combined effects of Bisphenol A and Benzophenone-3 on trophoblasts, mast cells, and their cellular crosstalk |
| Author | Schüffler, A.; Schumacher, A.; Zhang, N.; Zenclussen, M.L.; Howanski, J.; Zantop Linares, S.; Fink, B.; Bauer, M.; Zenclussen, A.C.; Meyer, N.; Kretschmer, T.
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| Source Titel | Journal of Hazardous Materials Advances |
| Year | 2026 |
| Department | IMMU |
| Language | englisch |
| Topic | T9 Healthy Planet |
| Supplements | Supplement 1 Supplement 2 |
| Keywords | endocrine disrupting chemicals; bisphenol A; benzophenone 3; trophoblast cells; mast cells; trophoblast-mast cell crosstalk |
| Abstract | Human exposure to
endocrine-disrupting chemicals (EDCs) is ubiquitous and has been
associated with adverse health effects, particularly during sensitive
developmental periods such as pregnancy. Experimental evidence with
single EDCs suggests impaired placentation and trophoblast function.
However, effects of combined EDC exposure on trophoblasts and maternal
immune cells, both pivotal during pregnancy, are largely unknown. Thus,
following on in vivo studies addressing the impact of single
and combined bisphenol A (BPA) and benzophenone-3 (BP-3) exposure on
pregnancy outcomes, this study sought to understand the effect of both
these EDCs on human trophoblasts and mast cells (MCs). We exposed
HTR-8/SVneo (trophoblasts) and HMC-1 (MCs) to physiological and
supraphysiological levels of BPA and BP-3 ranging from 0.001 µM to 10 µM
and performed various assays to determine cell viability, metabolic
activity, proliferation, trophoblast and transwell migration, and we
measured changes in cytokine levels and gene expression of relevant
marker genes for trophoblast function. Only a few isolated statistically
significant changes were observed, including a change in trophoblast
metabolic activity following BPA exposure and altered MC TIMP-1
secretion following combined BPA and BP-3 exposure. In contrast, no
statistically significant effects were detected on trophoblast or MC
viability, proliferation, trophoblast migration, MC IL-6 secretion, or
the expression of the investigated trophoblast-related genes.
Furthermore, chemical pre-exposure of either cell type did not
significantly affect trophoblast migration in co-culture. Our findings indicate that BPA and BP-3 had limited effects on the investigated cellular functions under the experimental conditions tested, without an apparent concentration-dependent pattern. This study extends our understanding of the cellular responses to BPA and BP-3 by examining both trophoblasts and MCs and their functional interactions, providing further insights into their potential relevance for placentation. |
| Schüffler, A., Schumacher, A., Zhang, N., Zenclussen, M.L., Howanski, J., Zantop Linares, S., Fink, B., Bauer, M., Zenclussen, A.C., Meyer, N., Kretschmer, T. (2026): Single and combined effects of Bisphenol A and Benzophenone-3 on trophoblasts, mast cells, and their cellular crosstalk J. Hazard. Mater. Adv. 10.1016/j.hazadv.2026.101705 |
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