Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.bios.2026.118805 |
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| Title (Primary) | A field-deployable immunofluorescence chip for adenovirus monitoring in groundwater systems |
| Author | Hossain, M.; Hastreiter, N.
; Vienken, T.; Wegmann, M.; Roggenbuck, D.; Engelhardt, I. |
| Source Titel | Biosensors & Bioelectronics |
| Year | 2026 |
| Department | MET |
| Volume | 308 |
| Page From | art. 118805 |
| Language | englisch |
| Topic | T8 Georesources |
| Supplements | Supplement 1 |
| Keywords | Adenovirus monitoring; Field-deployable immunofluorescence chip; Antibody-functionalized biosensor; Waterborne pathogen surveillance; Portable virus detection platform |
| Abstract | Rapid field detection of human adenoviruses in groundwater remains a critical technological gap, particularly for riverbank filtration systems, exposed to extreme weather and contamination events. Here, we present the VIRUMEX chip, a field-deployable platform that captures and detects adenovirus proteins through popular immunofluorescence mechanism using antibody-functionalized 3D Sepharose beads integrated into a disposable flow-through microfluidic cartridge. Powered by a compact peristaltic pump, the system autonomously processes groundwater and yields fluorescence-based readouts in light intensity unit (LIU) within 4–8 h, enabling near–real time semi-quantitative pathogen surveillance. Laboratory tests produced a clear detectable signal at 0.129 ng/mL (1000 LIU) for adenovirus hexon protein, and field deployment in the Erpe River catchment produced above-background fluorescence (800 LIU) in river water, providing proof-of-concept evidence with positive signal under real riverbank filtration conditions, while groundwater samples remained negative under the tested conditions. By providing an on-site, rapid, and operationally simple detection method, the VIRUMEX chip provides an additional on-site virus screening prototype alongside qPCR and isothermal approaches and offers a promising prototype for integrating virus monitoring into groundwater safety frameworks which needs some further optimizations before it can be regarded as a fully validated detection tool. |
| Hossain, M., Hastreiter, N., Vienken, T., Wegmann, M., Roggenbuck, D., Engelhardt, I. (2026): A field-deployable immunofluorescence chip for adenovirus monitoring in groundwater systems Biosens. Bioelectron. 308 , art. 118805 10.1016/j.bios.2026.118805 |
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