Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.aquatox.2026.107822 |
Licence  |
|
| Title (Primary) | Comparative effect assessment of bisphenol analogs by high content analysis in zebrafish (Danio rerio) embryos |
| Author | Aslam, M.A.
; Massei, R.; Scholz, S.
|
| Source Titel | Aquatic Toxicology |
| Year | 2026 |
| Department | MET; ETOX |
| Volume | 295 |
| Page From | art. 107822 |
| Language | englisch |
| Topic | T9 Healthy Planet T5 Future Landscapes |
| Data and Software links | https://doi.org/10.6019/S-BIAD3261 |
| Supplements | Supplement 1 Supplement 2 |
| Keywords | Zebrafish; Bisphenols; Toxicology; Risk assessment; Baseline toxicity |
| Abstract | Bisphenol
A (BPA), a plastic additive, has been banned from certain products,
particularly because of its endocrine-disrupting properties. As a
result, substitutes such as bisphenol E (BPE), bisphenol G (BPG),
bisphenol AP (BPAP), and bisphenol Z (BPZ) were developed, which may be
equally or even more bioactive. In this study, a comparative and
comprehensive assessment was conducted using a high-content screening
(HCS) assay, with zebrafish (Danio rerio) embryo, with a focus
on non-endocrine (neuro)developmental effects. In contrast to previous
analyses, the comparison was based on an automated, quantitative image
and video analysis approach, which reduces observer bias and increases
the reproducibility of results. Zebrafish embryos were exposed to 5
concentrations of each bisphenol, with predicted baseline toxicity as
the highest concentration, followed by a 2-fold serial dilution. The
(neuro)developmental effects were quantified by using behavior
endpoints, heart rate assessment and quantification of deviation of
morphological endpoints from controls. For all endpoints,
concentration-response modeling was applied to derive effect
concentrations. The specificity of responses was estimated by applying
the baseline toxicity and calculating effect ratios to observed
mortality. The effect profile of bisphenols was compared with two
presumable baseline toxicants (Diphenylamine and Methylaniline). Based
on the detailed assessment of BPA and its 4 substitutes, a screening
approach was developed to prioritize further BPA analogues for detailed
assessment. Our results show that the absence of the posterior
swimbladder was the most sensitive endpoint for all the tested
bisphenols, particularly for BPE. Comparison to baseline toxicity
indicated that the developmental effects were in the range of predicted
baseline toxicity for all bisphenols. |
| Aslam, M.A., Massei, R., Scholz, S. (2026): Comparative effect assessment of bisphenol analogs by high content analysis in zebrafish (Danio rerio) embryos Aquat. Toxicol. 295 , art. 107822 10.1016/j.aquatox.2026.107822 |
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