Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1007/s00253-026-13792-3 |
Licence  |
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| Title (Primary) | Physiological characterization of anaerobic cometabolic transformation of sulfamethoxazole by Nitratidesulfovibrio vulgaris Hildenborough |
| Author | Köpke, J.
; Ouyang, W.-Y.; Akay, C.
; Ding, C.; Ruhl, A.S.; Adrian, L.
|
| Source Titel | Applied Microbiology and Biotechnology |
| Year | 2026 |
| Department | MIBITECH; MEB |
| Volume | 110 |
| Page From | art. 129 |
| Language | englisch |
| Topic | T7 Bioeconomy |
| Data and Software links | https://doi.org/10.6019/PXD068774 |
| Supplements | Supplement 1 Supplement 2 |
| Keywords | Activity assay; Bioremediation; Comparative proteomics; Isoxazole ring; Redox differentiation; Retransformation; Sulfate reduction |
| Abstract | Sulfonamide antibiotics are widely used in medicine and farming and
residual amounts end up in wastewater effluents and solids applied as
fertilizers in agriculture. Residues could promote antibiotic resistance
propagation, especially during activated sludge treatment. We
previously reported as a proof of concept that Nitratidesulfovibrio vulgaris Hildenborough (NvH) (formerly Desulfovibrio vulgaris
Hildenborough) can transform the sulfonamide antibiotic
sulfamethoxazole (SMX) anaerobically. Here, we studied in detail the
influence of SMX on the physiology of NvH, the effect of different
electron donors on SMX transformation, and the stability of the
transformation products (TPs) under anoxic and oxic conditions. SMX
transformation was catalyzed by resting cells and no difference was
observed between acclimated and non-acclimated cells. Higher SMX
transformation activity was observed with higher initial SMX
concentrations and with younger cultures in exponential phase. SMX
transformation was supported in the presence of lactate, and slowed down
when lactate was depleted. When lactate was replaced with H2
plus acetate, sulfate reduction, cell growth, and SMX transformation
still took place. The expression pattern of key catabolic proteins was
unaltered by the presence of SMX. SMX was transformed by NvH to two
major TPs, TP253 and TP255. TP255 was identified as the reduced form of
SMX and was abiotically decaying under oxic conditions to TP187, which
no longer had an intact sulfonamide structure. Our results suggest that
SMX can be transformed under sulfate-reducing conditions by a sequence
of anaerobic microbial reduction and oxic abiotic decay, decreasing the
persistence and resistance propagation potential of SMX. |
| Köpke, J., Ouyang, W.-Y., Akay, C., Ding, C., Ruhl, A.S., Adrian, L. (2026): Physiological characterization of anaerobic cometabolic transformation of sulfamethoxazole by Nitratidesulfovibrio vulgaris Hildenborough Appl. Microbiol. Biotechnol. 110 , art. 129 10.1007/s00253-026-13792-3 |
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