Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1093/femsec/fiag005 |
Licence  |
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| Title (Primary) | The PceA-orthologous reductive dehalogenase of Dehalococcoides mccartyi CBDB1 is involved in 1,2,4-trichlorobenzene respiration |
| Author | Greiner-Haas, F.; Türkowsky, D.; Schwoch, S.; Bischler, T.; Jehmlich, N.
; von Bergen, M.; Förstner, K.; Sharma, C.M.; Sawers, G.; Goris, T.; Lechner, U. |
| Source Titel | FEMS Microbiology Ecology |
| Year | 2026 |
| Department | MOLTOX |
| Volume | 102 |
| Issue | 3 |
| Page From | fiag005 |
| Language | englisch |
| Topic | T9 Healthy Planet |
| Supplements | Supplement 1 |
| Keywords | Dehalococcoides mccartyi; reductive dehalogenase; transcriptome; proteome; trichlorobenzene; PceA; sRNA |
| Abstract | The reductive dehalogenation of halogenated benzenes by anaerobic bacteria is of great environmental and biotechnological importance; however, the role of specific reductive dehalogenases in the dehalogenation of different isomers has not been studied in detail. Here, we cultivated the obligate organohalide-respiring Dehalococcoides mccartyi strain CBDB1 with either 1,2,3- or 1,2,4-trichlorobenzene (TCB) as electron acceptor and investigated the transcription of its 32 reductive dehalogenase (rdhA) genes using RNA-sequencing. The chlorobenzene reductive dehalogenase gene cbrA, and rdhA cbdbA80 were the two most highly expressed rdhA genes with 1,2,3-TCB. In the presence of 1,2,4-TCB, cbrA was the most highly expressed rdhA followed by rdhA cbdbA1588, encoding an orthologue of the tetrachloroethene reductive dehalogenase PceA of D. mccartyi strain 195. RNA-sequencing also allowed for the detection of small RNAs and an unannotated protein. Proteomics confirmed the synthesis of RdhA CbdbA1588 during respiration with 1,2,4-TCB and also with hexachlorobenzene, which is dehalogenated via 1,2,4-TCB. Dehalogenase activity assays with cell extracts from 1,2,4-TCB-grown cultures indicated a higher activity towards 1,2,4-TCB and a ten-fold higher activity towards 2,3-dichlorophenol compared to that in extracts from 1,2,3-TCB-grown cultures. These findings demonstrate the functionality of RdhA CbdbA1588 and further support a role in 1,2,4-TCB dechlorination by strain CBDB1. |
| Greiner-Haas, F., Türkowsky, D., Schwoch, S., Bischler, T., Jehmlich, N., von Bergen, M., Förstner, K., Sharma, C.M., Sawers, G., Goris, T., Lechner, U. (2026): The PceA-orthologous reductive dehalogenase of Dehalococcoides mccartyi CBDB1 is involved in 1,2,4-trichlorobenzene respiration FEMS Microbiol. Ecol. 102 (3), fiag005 10.1093/femsec/fiag005 |
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